超越编辑，迈向书写大型基因组。
Beyond editing to writing large genomes.
作者信息
Chari Raj, Church George M
机构信息
Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts, 02115, USA.
Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts, 02115, USA, and at the Wyss Institute for Biologically Inspired Engineering, 3 Blackfan Circle, Boston, Massachusetts, 02115, USA.
出版信息
Nat Rev Genet. 2017 Dec;18(12):749-760. doi: 10.1038/nrg.2017.59. Epub 2017 Aug 30.
Abstract
Recent exponential advances in genome sequencing and engineering technologies have enabled an unprecedented level of interrogation into the impact of DNA variation (genotype) on cellular function (phenotype). Furthermore, these advances have also prompted realistic discussion of writing and radically re-writing complex genomes. In this Perspective, we detail the motivation for large-scale engineering, discuss the progress made from such projects in bacteria and yeast and describe how various genome-engineering technologies will contribute to this effort. Finally, we describe the features of an ideal platform and provide a roadmap to facilitate the efficient writing of large genomes.
摘要
基因组测序和工程技术最近呈指数级发展，使得人们能够以前所未有的深度探究DNA变异（基因型）对细胞功能（表型）的影响。此外，这些进展也引发了关于编写和彻底重写复杂基因组的现实讨论。在这篇观点文章中，我们详细阐述了大规模工程的动机，讨论了在细菌和酵母中此类项目所取得的进展，并描述了各种基因组工程技术将如何助力这一努力。最后，我们描述了理想平台的特征，并提供了一个路线图以促进大型基因组的高效编写。
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