基因组编写：当前进展与相关应用。

Genome Writing: Current Progress and Related Applications.

机构信息

BGI-Shenzhen, Shenzhen 518083, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518083, China; Shenzhen Engineering Laboratory for Innovative Molecular Diagnostics, BGI-Shenzhen, Shenzhen 518083, China; Guangdong Provincial Key Laboratory of Genome Read and Write, Shenzhen 518120, China.

BGI-Shenzhen, Shenzhen 518083, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518083, China; BGI-Qingdao, Qingdao 266555, China; Shenzhen Engineering Laboratory for Innovative Molecular Diagnostics, BGI-Shenzhen, Shenzhen 518083, China; Guangdong Provincial Key Laboratory of Genome Read and Write, Shenzhen 518120, China.

出版信息

Genomics Proteomics Bioinformatics. 2018 Feb;16(1):10-16. doi: 10.1016/j.gpb.2018.02.001. Epub 2018 Feb 21.

DOI:10.1016/j.gpb.2018.02.001

PMID:29474887

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6000237/

Abstract

The ultimate goal of synthetic biology is to build customized cells or organisms to meet specific industrial or medical needs. The most important part of the customized cell is a synthetic genome. Advanced genomic writing technologies are required to build such an artificial genome. Recently, the partially-completed synthetic yeast genome project represents a milestone in this field. In this mini review, we briefly introduce the techniques for de novo genome synthesis and genome editing. Furthermore, we summarize recent research progresses and highlight several applications in the synthetic genome field. Finally, we discuss current challenges and future prospects.

摘要

合成生物学的最终目标是构建定制化的细胞或生物体，以满足特定的工业或医学需求。定制化细胞的最重要部分是一个合成基因组。构建这样的人工基因组需要先进的基因组编写技术。最近，部分完成的合成酵母基因组项目代表了该领域的一个里程碑。在这篇迷你综述中，我们简要介绍了从头合成基因组和基因组编辑的技术。此外，我们总结了最近的研究进展，并强调了合成基因组领域的几个应用。最后，我们讨论了当前的挑战和未来的前景。