Shimetani Naoto
International University of Health and Welfare Atami Hospital.
Med Mycol J. 2017;58(3):J91-J94. doi: 10.3314/mmj.17.012.
Real-time test results are necessary for early diagnosis and in determining treatment orientation in medical practice. Point of Care Testing (POCT) is a testing system that provides beneficial and helpful information for diagnosis and treatment through real-time testing at the bedside. Therefore, POCT has high utility value in the field of infectious diseases as a rapid test that provides, within the consultation hours, useful information for initial treatment.Infectious disease rapid test kits are commercially available for a wide variety of prophlogistic pathogen targets, including bacterial, viral, fungal, protozoal, and other disease agents. One of these kits is immunochromatography assay (ICA), a measuring method used as POCT that is easy to operate, wherein even physicians and nurses can conduct the test.Serodiagnostic method has been adjunctively used in medical practice in Japan for early clinical diagnosis of deep mycosis as a means to determine treatments. However, this method is complicated and is considered a full-scale clinical examination; therefore, it is not included in the category of POCT. Recently, Loop-Mediated Isothermal Amplification (LAMP) method has been tested as a POCT for diagnosis of fungal infection in the US. Development of a laboratory procedure using simple and highly accurate POCT for early diagnosis of deep mycosis is expected in the near future.
在医学实践中，实时检测结果对于早期诊断和确定治疗方向至关重要。即时检验（POCT）是一种通过在床边进行实时检测为诊断和治疗提供有益信息的检测系统。因此，POCT作为一种能在会诊时间内为初始治疗提供有用信息的快速检测方法，在传染病领域具有很高的实用价值。传染病快速检测试剂盒可用于多种致病病原体靶点，包括细菌、病毒、真菌、原生动物和其他病原体。其中一种试剂盒是免疫层析法（ICA），这是一种用作POCT的测量方法，操作简便，甚至医生和护士都可以进行检测。血清诊断方法在日本的医学实践中已被辅助用于深部真菌病的早期临床诊断，作为确定治疗方法的手段。然而，这种方法很复杂，被视为全面的临床检查；因此，它不属于POCT类别。最近，环介导等温扩增（LAMP）法已在美国作为诊断真菌感染的POCT进行测试。预计在不久的将来会开发出一种使用简单且高度准确的POCT进行深部真菌病早期诊断的实验室程序。
