Jin Zaishun, Zhan Tao, Tao Jing, Xu Biao, Zheng Huizhe, Cheng Yongxia, Yan Bin, Wang Hongwei, Lu Guoqiang, Lin Ying, Guo Sufen
a Key Laboratory of Cancer Prevention and Treatment of Heilongjiang Province , Mudanjiang Medical University , Mudanjiang , People's Republic of China.
b Department of Pathology , Mudanjiang Medical University , Mudanjiang , People's Republic of China.
Biosci Biotechnol Biochem. 2017 Oct;81(10):1899-1907. doi: 10.1080/09168451.2017.1364965. Epub 2017 Sep 1.
The function of microRNA-34a (miR-34a) in transdifferentiation of glioma stem cells (GSCs) into vascular endothelial cells (VECs) was explored by focusing on Notch ligand Delta-like 1 (Dll1). MiR-34a mimics was transfected into CD133 + glioma cell U251. The angiogenesis feature of miR-34a transfected U251 cells was investigated and the expressions of CD31, CD34, Vwf, Notch 1, and Dll1 were quantified. Length of branching vessel-like structures in the miR-34a transfected U251 cells was significantly higher than control cells. The VEC feature of miR-34a overexpressed U251 cells was further confirmed by the expressions of CD31, CD34, and vWF. Transfection of miR-34a decreased the expression of Notch 1 and Dll1. Furthermore, the miR-34a overexpression-enhanced tube formation of GSCs was suppressed when the decreased expression of Dll1 was restored. The current study highlighted the potential of miR-34a as an inducer in GSCs' transdifferentiation into VECs by targeting Dll1.
通过聚焦Notch配体Delta样1（Dll1），探讨了微小RNA-34a（miR-34a）在胶质瘤干细胞（GSCs）向血管内皮细胞（VECs）转分化中的作用。将miR-34a模拟物转染到CD133 +胶质瘤细胞U251中。研究了转染miR-34a的U251细胞的血管生成特征，并对CD31、CD34、血管性血友病因子（Vwf）、Notch 1和Dll1的表达进行了定量分析。转染miR-34a的U251细胞中分支状血管样结构的长度显著高于对照细胞。CD31、CD34和vWF的表达进一步证实了过表达miR-34a的U251细胞具有VEC特征。miR-34a转染降低了Notch 1和Dll1的表达。此外，当Dll1表达降低得到恢复时，miR-34a过表达增强的GSCs管腔形成受到抑制。当前研究突出了miR-34a通过靶向Dll1作为GSCs向VECs转分化诱导剂的潜力。
