Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, 411007, India.
Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, 411007, India; Department of Microbiology, Savitribai Phule Pune University, Pune, 411007, India.
Enzyme Microb Technol. 2017 Nov;106:48-54. doi: 10.1016/j.enzmictec.2017.07.002. Epub 2017 Jul 8.
Controlling disease outbreaks is a major challenge in aquaculture farms and conventional methods are often ineffective. Nutritional supplementation and probiotic preparations help in reducing severity of such infections. The generally regarded as safe yeast (Yarrowia lipolytica) was used in the current study. A marine strain of Y. lipolytica exhibited tolerance towards sodium selenite and formed cell associated selenium nanoparticles (SeNPs). The synthesized nanoparticles were characterized by UV-vis spectroscopy, X-ray diffraction (XRD) and Field Emission Scanning Electron Microscope (FE-SEM) observations. Fourier transform infra-red (FTIR) spectroscopy indicated the role of carboxylic and amine groups in the synthesis of nanoparticles. This SeNP-enriched biomass was used as feed for the model aquaculture system, Artemia salina and compared with normal feed, baker's yeast (Saccharomyces cerevisiae). A. salina fed with SeNP-enriched biomass, showed increased survival rates (96.66%) as compared to those fed with S. cerevisiae (60.0%). The size of the larvae fed with SeNP-enriched biomass of Y. lipolytica was also found to be larger. Additionally, larval groups fed with SeNP-enriched biomass were better protected (70.0% survival) against V. harveyi infection when compared with groups fed with S. cerevisiae (24.44%). This combination of selenium in the nanoparticle form associated with the biomass of Y. lipolytica has potential application in improving health of aquaculture species in farms.
控制水产养殖中的疾病爆发是一个重大挑战，传统方法往往效果不佳。营养补充和益生菌制剂有助于减轻此类感染的严重程度。本研究中使用了公认安全的酵母（Yarrowia lipolytica）。海洋 Y. lipolytica 菌株对亚硒酸钠具有耐受性，并形成细胞相关的硒纳米颗粒（SeNPs）。合成的纳米颗粒通过紫外-可见光谱、X 射线衍射（XRD）和场发射扫描电子显微镜（FE-SEM）观察进行了表征。傅里叶变换红外（FTIR）光谱表明，羧酸和胺基在纳米颗粒的合成中发挥了作用。这种富含 SeNP 的生物质被用作模式水产养殖系统（卤虫）的饲料，并与普通饲料（面包酵母）进行了比较。与喂食 S. cerevisiae（酿酒酵母）的卤虫相比，喂食富含 SeNP 的 Y. lipolytica 生物质的卤虫存活率（96.66%）更高。喂食富含 SeNP 的 Y. lipolytica 生物质的幼虫的大小也更大。此外，与喂食 S. cerevisiae 的幼虫组（70.0%的存活率）相比，喂食富含 SeNP 的生物质的幼虫组在对抗 V. harveyi 感染时受到了更好的保护（70.0%的存活率）。这种与 Y. lipolytica 生物质结合的纳米形式的硒可能具有改善养殖场中水产养殖物种健康的应用潜力。
