School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Marketing Department, Leading Pharm Medical Technology, Inc., Beijing 100083, China.
Molecules. 2017 Aug 30;22(9):1425. doi: 10.3390/molecules22091425.
Processing is a traditional pharmacy technology based on traditional Chinese medicine theory. The traditional Chinese medicine (TCM) ingredients should be processed before being used as a medicine. Processed bitter almonds are widely used in the clinic in TCM for the treatment of cough and asthma. In this work the amygdalin profile of three producing areas in China was determined, with respect to three differently processed bitter almond products: raw, stir-fried and scalded. Identification of the compounds was done by using high performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS/MS). Results indicated that amygdalin, neoamygdalin and amygdalin amide were identified in the different processed bitter almonds. Meanwhile, amygdalin was used as a standard to calculate the quantification of amygdalin and the concentration ratio of neoamygdalin and total amygdalin by HPLC-DAD. The data suggested that composition of amygdalin isomers in bitter almonds was influenced by the processing method. It also gives a new understanding of the processing principle of bitter almonds. Moreover, the classification of different processed bitter almonds can be achieved on the basis of amygdalin isomers levels.
炮制是一种基于中医药理论的传统制药技术。中药材在用作药物之前应进行炮制。炮制苦杏仁在中医药临床中广泛用于治疗咳嗽和哮喘。在这项工作中，测定了中国三个产地的三种不同炮制苦杏仁产品的苦杏仁苷谱：生、炒和烫。通过高效液相色谱-电喷雾电离质谱联用（HPLC-ESI-MS/MS）进行化合物的鉴定。结果表明，不同炮制苦杏仁中均鉴定出苦杏仁苷、新苦杏仁苷和苦杏仁酰胺。同时，以苦杏仁苷为标准，采用 HPLC-DAD 计算苦杏仁苷的定量和新苦杏仁苷与总苦杏仁苷的浓度比。数据表明，苦杏仁中苦杏仁苷异构体的组成受炮制方法的影响。这也为苦杏仁的炮制原理提供了新的认识。此外，可以基于苦杏仁苷异构体水平对不同炮制苦杏仁进行分类。
