College of Pharmacy and Chemistry & Chemical Engineering, Taizhou University, Taizhou 225300, China.
Molecules. 2024 Oct 11;29(20):4801. doi: 10.3390/molecules29204801.
Cyanogenic glycosides are naturally occurring compounds found in numerous plant species, which can release toxic hydrogen cyanide upon hydrolysis. The quantification of cyanogenic glycosides is essential for assessing their potential toxicity and health risks associated with their consumption. Liquid chromatographic techniques coupled with various detectors have been widely used for the quantification of cyanogenic glycosides. In this review, we discuss recent advances in chromatographic quantification methods for cyanogenic glycosides, including the development of new stationary phases, innovative sample preparation methods, and the use of mass spectrometry. We also highlight the combination of chromatographic separation with mass spectrometric detection for the identification and quantification of specific cyanogenic glycosides and their metabolites in complex sample matrices. Lastly, we discuss the current challenges and future perspectives in the development of reliable reference standards, optimization of sample preparation methods, and establishment of robust quality control procedures. This review aims to provide an overview of recent advances in chromatographic quantification methods for cyanogenic glycosides and their applications in various matrices, including food products, biological fluids, and environmental samples.
氰苷是存在于许多植物物种中的天然化合物，在水解时会释放出有毒的氰化氢。定量分析氰苷对于评估其潜在毒性以及与食用相关的健康风险至关重要。液相反相色谱技术与各种检测器已广泛用于氰苷的定量分析。本综述讨论了氰苷色谱定量方法的最新进展，包括新型固定相的开发、创新的样品制备方法以及质谱的应用。我们还强调了色谱分离与质谱检测相结合，用于鉴定和定量复杂样品基质中特定氰苷及其代谢物的方法。最后，我们讨论了在开发可靠的参考标准、优化样品制备方法以及建立稳健的质量控制程序方面所面临的挑战和未来展望。本综述旨在概述用于氰苷的色谱定量方法的最新进展及其在各种基质中的应用，包括食品、生物体液和环境样品。
