Mao Kun-Jun, Huang Ping, Zhang Li-, Yu Hai-Sheng, Zhou Xing-Zhuo, Ye Xi-Yong
a Department of Pharmacy , Jiangxi Medical College , Shangrao , PR China.
b The Affiliated Hospital of Qingdao University , Qingdao , PR China.
Nat Prod Res. 2018 Sep;32(18):2233-2237. doi: 10.1080/14786419.2017.1371152. Epub 2017 Sep 5.
Cynanchum wallichii Wight, is a traditional Chinese medicine herb, which is rich in saponins and has varieties of pharmacological activities. In this study, a standardized C. wallichii extract was established and the anti-tumor activity of the total saponins was evaluated by MTT assay. The extraction conditions of the standardized extract was optimized using response surface methodology. The experimental value was in good agreement (the yield 4.28%) with predicted values. The total saponins of the extract showed significant anti-tumor activity against three human tumor cell lines (A549, HepG2 and MCF-7), especially for MCF-7 (IC. 67.63 μg/mL) cells in vitro.
白首乌是一种传统的中草药，富含皂苷，具有多种药理活性。本研究制备了标准化的白首乌提取物，并通过MTT法评估了总皂苷的抗肿瘤活性。采用响应面法对白首乌标准化提取物的提取条件进行了优化。实验值（产率4.28%）与预测值吻合良好。该提取物的总皂苷在体外对三种人类肿瘤细胞系（A549、HepG2和MCF-7）表现出显著的抗肿瘤活性，尤其是对MCF-7细胞（IC50为67.63μg/mL）。
