Key Laboratory of Xi'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei 230038, China.
Research and Technology Center, Anhui University of Chinese Medicine, Hefei 230038, China.
Molecules. 2022 Aug 31;27(17):5627. doi: 10.3390/molecules27175627.
Cotton is one of the Uyghur medical materials in China and is rich in flavonoids. Flavonoids have important pharmacological effects. The yield of flavonoids in traditional extraction methods is low, which affects the development of flavonoids. Therefore, it is urgent to optimize the extraction techniques. The yield of flavonoids in cotton flowers was effectively improved by response surface methodology, and the highest yield of flavonoids reached 5.66%, and the optimal extraction process conditions were obtained. The DPPH free radical scavenging rate, hydroxyl free radical scavenging rate, superoxide anion free radical scavenging rate, and reducing ability were tested to reflect the antioxidant capacity of flavonoids. The flavonoids had an excellent antioxidant effect. Cell experiments suggested that the flavonoids had the effect of protecting glutamate-induced damage to HT-22 cells. The results of this study provide a theoretical basis for the extraction of cotton flowers flavonoids and the comprehensive evaluation of antioxidant products, as well as the extraction of other plant flavonoids.
棉花是中国维吾尔族医学中的药用材料之一，富含黄酮类化合物。黄酮类化合物具有重要的药理作用。传统提取方法中黄酮类化合物的产率较低，影响了黄酮类化合物的发展。因此，迫切需要优化提取技术。利用响应面法有效提高了棉花花中黄酮类化合物的产率，黄酮类化合物的最高产率达到 5.66%，并获得了最佳的提取工艺条件。通过 DPPH 自由基清除率、羟基自由基清除率、超氧阴离子自由基清除率和还原能力来反映黄酮类化合物的抗氧化能力。黄酮类化合物具有极好的抗氧化作用。细胞实验表明，黄酮类化合物具有保护 HT-22 细胞免受谷氨酸损伤的作用。本研究为棉花花黄酮类化合物的提取和抗氧化产品的综合评价以及其他植物黄酮类化合物的提取提供了理论依据。
