Mosselhy Dina A, Granbohm Henrika, Hynönen Ulla, Ge Yanling, Palva Airi, Nordström Katrina, Hannula Simo-Pekka
Department of Chemistry and Materials Science, School of Chemical Engineering, Aalto University, 02150 Espoo, Finland.
Microbiological Unit, Fish Diseases Department, Animal Health Research Institute, Dokki, Giza 12618, Egypt.
Nanomaterials (Basel). 2017 Sep 6;7(9):261. doi: 10.3390/nano7090261.
Infected superficial wounds were traditionally controlled by topical antibiotics until the emergence of antibiotic-resistant bacteria. Silver (Ag) is a kernel for alternative antibacterial agents to fight this resistance quandary. The present study demonstrates a method for immobilizing small-sized (~5 nm) silver nanoparticles on silica matrix to form a nanosilver-silica (Ag-SiO₂) composite and shows the prolonged antibacterial effects of the composite in vitro. The composite exhibited a rapid initial Ag release after 24 h and a slower leaching after 48 and 72 h and was effective against both methicillin-resistant (MRSA) and ( . ). Ultraviolet (UV)-irradiation was superior to filter-sterilization in retaining the antibacterial effects of the composite, through the higher remaining Ag concentration. A gauze, impregnated with the Ag-SiO₂ composite, showed higher antibacterial effects against MRSA and . than a commercial Ag-containing dressing, indicating a potential for the management and infection control of superficial wounds. Transmission and scanning transmission electron microscope analyses of the composite-treated MRSA revealed an interaction of the released silver ions with the bacterial cytoplasmic constituents, causing ultimately the loss of bacterial membranes. The present results indicate that the Ag-SiO₂ composite, with prolonged antibacterial effects, is a promising candidate for wound dressing applications.
在耐抗生素细菌出现之前，感染的浅表伤口传统上通过局部使用抗生素来控制。银（Ag）是对抗这种耐药困境的替代抗菌剂的核心。本研究展示了一种将小尺寸（约5纳米）银纳米颗粒固定在二氧化硅基质上以形成纳米银 - 二氧化硅（Ag - SiO₂）复合材料的方法，并展示了该复合材料在体外的长效抗菌作用。该复合材料在24小时后初始银释放迅速，在48小时和72小时后浸出较慢，并且对耐甲氧西林金黄色葡萄球菌（MRSA）和（此处原文缺失部分内容）均有效。紫外线（UV）照射在保持复合材料抗菌效果方面优于过滤除菌，因为剩余银浓度更高。浸有Ag - SiO₂复合材料的纱布对MRSA和（此处原文缺失部分内容）的抗菌效果高于市售含银敷料，表明其在浅表伤口管理和感染控制方面具有潜力。对经复合材料处理的MRSA进行透射和扫描透射电子显微镜分析显示，释放的银离子与细菌细胞质成分相互作用，最终导致细菌细胞膜受损。目前的结果表明，具有长效抗菌作用的Ag - SiO₂复合材料是伤口敷料应用的有前途的候选材料。
