Singh Ashish Kumar, Prakash Pradyot, Achra Arvind, Singh Gyan Prakash, Das Arghya, Singh Rakesh Kumar
Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Department of Community Medicine, Division of Biostatistics, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
J Glob Infect Dis. 2017 Jul-Sep;9(3):93-101. doi: 10.4103/jgid.jgid_91_16.
is Gram-positive bacterium commonly associated with nosocomial infections. The development of biofilm exhibiting drug resistance especially in foreign body associated infections has enabled the bacterium to draw considerable attention. However, till date, consensus guidelines for biofilm quantitation and categorization criterion for the bacterial isolates based on biofilm-forming capacity are lacking. Therefore, it was intended to standardize biofilm formation by clinical isolates of and then to classify them on the basis of their biofilm-forming capacity.
A study was conducted for biofilm quantitation by tissue culture plate (TCP) assay employing 61 strains of isolated from clinical samples during May 2015- December 2015 wherein several factors influencing the biofilm formation were optimized. Therefore, it was intended to propose a biofilm classification criteria based on the standard deviation multiples of the control differentiating them into non, low, medium, and high biofilm formers.
Brain-heart infusion broth was found to be more effective in biofilm formation compared to trypticase soy broth. Heat fixation was more effective than chemical fixation. Although, individually, glucose, sucrose, and sodium chloride (NaCl) had no significant effect on biofilm formation, a statistically significant increase in absorbance was observed after using the supplement mix consisting of 222.2 mM glucose, 116.9 mM sucrose, and 1000 mM NaCl (= 0.037).
The present study puts forth a standardized TCP assay for biofilm biomass quantitation and categorization criteria for clinical isolates of based on their biofilm-forming capacity. The proposed technique may be further evaluated for its usefulness in the management of persistent infections caused by the bacterium.
[细菌名称]是一种革兰氏阳性菌，常与医院感染相关。生物膜的形成表现出耐药性，尤其是在与异物相关的感染中，这使得该细菌备受关注。然而，迄今为止，缺乏关于生物膜定量以及基于生物膜形成能力对细菌分离株进行分类标准的共识指南。因此，旨在规范[细菌名称]临床分离株的生物膜形成，然后根据其生物膜形成能力对它们进行分类。
进行了一项研究，采用2015年5月至2015年12月期间从临床样本中分离出的61株[细菌名称]，通过组织培养板（TCP）试验对生物膜进行定量，其中优化了影响生物膜形成的几个因素。因此，旨在提出一种基于对照标准差倍数的生物膜分类标准，将它们分为无、低、中、高生物膜形成者。
发现脑心浸液肉汤在生物膜形成方面比胰蛋白酶大豆肉汤更有效。热固定比化学固定更有效。虽然单独来看，葡萄糖、蔗糖和氯化钠（NaCl）对生物膜形成没有显著影响，但在使用由222.2 mM葡萄糖、116.9 mM蔗糖和1000 mM NaCl组成的补充混合物后，观察到吸光度有统计学上的显著增加（= 0.037）。
本研究提出了一种标准化的TCP试验，用于生物膜生物量定量以及基于生物膜形成能力对[细菌名称]临床分离株进行分类的标准。所提出的[细菌名称]技术在该细菌引起的持续性感染管理中的有用性可能需要进一步评估。
