Ahmed Ahmed El-Abd, Abd-Elmawgood Eman Ahmed, Hassan Mohammed H
Associate Professor, Department of Paediatrics, Faculty of Medicine, South Valley University, Qena, Egypt.
Lecturer, Department of Paediatrics, Faculty of Medicine, South Valley University, Qena, Egypt.
J Clin Diagn Res. 2017 Jul;11(7):BC17-BC21. doi: 10.7860/JCDR/2017/29085.10310. Epub 2017 Jul 1.
Information about oxidative stress in preterms with Respiratory Distress Syndrome (RDS) is defective, so various researches in this area are required, which may open new roads in understanding the pathogenesis of the disease, hence provide additional helpful therapeutic approaches.
To assess and compare the plasma level of protein carbonyls as a marker for oxidant status and the antioxidant enzymes; Superoxide Dismutase (SOD) and Glutathione Peroxidase (GPx) and the related trace minerals in the form of Copper (Cu), Zinc (Zn) and Selenium (Se) as markers for antioxidant status, in preterms with and without RDS.
A hospital-based case-control study was conducted on fifty-seven preterm neonates (37 preterms with RDS and 20 preterms without RDS) admitted to neonatal intensive care unit of Qena University Hospitals after approval of the University Hospital Ethical Committee. Plasma protein carbonyls assay was done using commercially available ELISA assay kit. Plasma Cu, Zn, Se, erythrocyte SOD and GPx activities assays were done using commercially available colorimetric assay kits.
Significant higher plasma levels of protein carbonyls and oxidant/antioxidants ratio (protein carbonyls/{SOD+GPx}) with significant lower plasma levels of Zn, Cu, Se, erythrocyte SOD and GPx activities were found in the preterms with RDS when compared with the preterms without RDS (p<0.001 for all measured markers for both groups). In terms of birth weights and gestational ages, they were negatively correlated with both plasma protein carbonyls and oxidant/antioxidants ratio and positively correlated with plasma copper, zinc, selenium, erythrocyte SOD and GPx activities in a statistically significant manner. Non-significant correlations were found between the measured oxidative stress markers and the severity of RDS.
Oxidative stress may have a contributory role in the development of RDS among preterms. Lower birth weight and prematurity may increase the susceptibity to oxidative stress among such patients.
关于患有呼吸窘迫综合征（RDS）的早产儿氧化应激的信息尚不明确，因此需要在该领域开展各种研究，这可能为理解该疾病的发病机制开辟新途径，从而提供更多有益的治疗方法。
评估并比较患有和未患有RDS的早产儿血浆中蛋白质羰基水平（作为氧化状态标志物）、抗氧化酶超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）以及作为抗氧化状态标志物的相关微量矿物质铜（Cu）、锌（Zn）和硒（Se）的水平。
在基纳大学医院新生儿重症监护病房收治的57例早产儿（37例患有RDS的早产儿和20例未患有RDS的早产儿）中开展了一项基于医院的病例对照研究，该研究经大学医院伦理委员会批准。使用市售ELISA检测试剂盒进行血浆蛋白质羰基检测。使用市售比色法检测试剂盒进行血浆铜、锌、硒、红细胞SOD和GPx活性检测。
与未患RDS的早产儿相比，患RDS的早产儿血浆蛋白质羰基水平和氧化/抗氧化剂比率（蛋白质羰基/{SOD+GPx}）显著更高，而血浆锌、铜、硒水平、红细胞SOD和GPx活性显著更低（两组所有检测标志物的p值均<0.001）。就出生体重和胎龄而言，它们与血浆蛋白质羰基和氧化/抗氧化剂比率呈负相关，与血浆铜、锌、硒、红细胞SOD和GPx活性呈正相关，且具有统计学意义。在检测的氧化应激标志物与RDS严重程度之间未发现显著相关性。
氧化应激可能在早产儿RDS的发生中起作用。较低的出生体重和早产可能会增加此类患者对氧化应激的易感性。
