Kirchberger Stefanie, Sturtzel Caterina, Pascoal Susana, Distel Martin
St. Anna Kinderkrebsforschung, Children's Cancer Research Institute, Innovative Cancer Models, Vienna, Austria.
Front Oncol. 2017 Aug 28;7:186. doi: 10.3389/fonc.2017.00186. eCollection 2017.
Over the last decade, zebrafish has proven to be a powerful model in cancer research. Zebrafish form tumors that histologically and genetically resemble human cancers. The live imaging and cost-effective compound screening possible with zebrafish especially complement classic mouse cancer models. Here, we report recent progress in the field, including genetically engineered zebrafish cancer models, xenotransplantation of human cancer cells into zebrafish, promising approaches toward live investigation of the tumor microenvironment, and identification of therapeutic strategies by performing compound screens on zebrafish cancer models. Given the recent advances in genome editing, personalized zebrafish cancer models are now a realistic possibility. In addition, ongoing automation will soon allow high-throughput compound screening using zebrafish cancer models to be part of preclinical precision medicine approaches.
在过去十年中，斑马鱼已被证明是癌症研究中的一种强大模型。斑马鱼形成的肿瘤在组织学和遗传学上与人类癌症相似。斑马鱼具备的活体成像和经济高效的化合物筛选能力，特别补充了经典的小鼠癌症模型。在此，我们报告该领域的最新进展，包括基因工程斑马鱼癌症模型、将人类癌细胞异种移植到斑马鱼中、对肿瘤微环境进行活体研究的有前景方法，以及通过对斑马鱼癌症模型进行化合物筛选来确定治疗策略。鉴于基因组编辑的最新进展，个性化斑马鱼癌症模型现在已成为现实的可能性。此外，正在进行的自动化将很快使使用斑马鱼癌症模型进行的高通量化合物筛选成为临床前精准医学方法的一部分。
