Na Takuathung Mingkwan, Wongnoppavich Ariyaphong, Pitchakarn Pornsiri, Panthong Ampai, Khonsung Parirat, Chiranthanut Natthakarn, Soonthornchareonnon Noppamas, Sireeratawong Seewaboon
Department of Pharmacology, Doctor of Philosophy Program in Pharmacology, Faculty of Medicine and Graduate School, Chiang Mai University, Muang 50200, Thailand.
Department of Pharmacology, Faculty of Medicine, Chiang Mai University, Muang, Chiang Mai 50200, Thailand.
Evid Based Complement Alternat Med. 2017;2017:5906539. doi: 10.1155/2017/5906539. Epub 2017 Aug 16.
Psoriasis is a chronic inflammatory and immune-mediated skin disease. The pathogenesis involves T cells activation via the IL-23/Th17 axis. Conventional treatments of psoriasis have adverse events influencing patients' adherence. Wannachawee Recipe (WCR) has been effectively used as Thai folk remedy for psoriasis patients; however, preclinical evidence defining how WCR works is still lacking. This study defined mechanisms for its antiproliferation and anti-inflammatory effects in HaCaT cells. The cytotoxicity and antiproliferation results from SRB and CCK-8 assays showed that WCR inhibited the growth and viability of HaCaT cells in a concentration-dependent manner. The distribution of cell cycle phases determined by flow cytometry showed that WCR did not interrupt cell cycle progression. Interestingly, RT-qPCR revealed that WCR significantly decreased the mRNA expression of IL-1, IL-6, IL-8, IL-17A, IL-22, IL-23, and TNF- but induced IL-10 expression in TNF-- and IFN--induced HaCaT cells. At the protein level determined by ELISA, WCR significantly reduced the secretion of IL-17A, IL-22, and IL-23. The WCR at low concentrations was proved to possess anti-inflammatory effect without cytotoxicity and it did not interfere with cell cycle of keratinocytes. This is the first study to provide convincing evidence that WCR is a potential candidate for development of effective psoriasis therapies.
银屑病是一种慢性炎症性和免疫介导的皮肤病。其发病机制涉及通过白细胞介素-23/辅助性T细胞17（IL-23/Th17）轴激活T细胞。银屑病的传统治疗存在不良事件，影响患者的依从性。万纳查维方（WCR）已被有效地用作泰国治疗银屑病患者的民间疗法；然而，仍缺乏确定WCR作用机制的临床前证据。本研究确定了其在人永生化角质形成细胞（HaCaT细胞）中的抗增殖和抗炎作用机制。磺酰罗丹明B（SRB）和细胞计数试剂盒-8（CCK-8）检测的细胞毒性和抗增殖结果表明，WCR以浓度依赖的方式抑制HaCaT细胞的生长和活力。流式细胞术测定的细胞周期阶段分布表明，WCR不干扰细胞周期进程。有趣的是，逆转录定量聚合酶链反应（RT-qPCR）显示，WCR显著降低了白细胞介素-1（IL-1）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、白细胞介素-17A（IL-17A）、白细胞介素-22（IL-22）、白细胞介素-23（IL-23）和肿瘤坏死因子-α（TNF-α）的信使核糖核酸（mRNA）表达，但在肿瘤坏死因子-α和干扰素-γ诱导的HaCaT细胞中诱导白细胞介素-10表达。酶联免疫吸附测定（ELISA）确定的蛋白质水平显示，WCR显著降低了IL-17A、IL-22和IL-23的分泌。低浓度的WCR被证明具有抗炎作用且无细胞毒性，并且不干扰角质形成细胞的细胞周期。这是第一项提供令人信服的证据表明WCR是开发有效银屑病治疗方法的潜在候选物的研究。
