Na Takuathung Mingkwan, Wongnoppavich Ariyaphong, Panthong Ampai, Khonsung Parirat, Chiranthanut Natthakarn, Soonthornchareonnon Noppamas, Sireeratawong Seewaboon
Department of Pharmacology, Faculty of Medicine and Graduate School, Chiang Mai University, Muang 50200, Thailand.
Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Muang, Chiang Mai 50200, Thailand.
Evid Based Complement Alternat Med. 2018 Jan 24;2018:7931031. doi: 10.1155/2018/7931031. eCollection 2018.
Psoriasis is a common immune-mediated chronic inflammatory skin disease characterized by thick and erythema raised plaques with adherent silvery scales. T-cells are activated via the IL-23/Th17 axis which is involved in psoriasis pathogenesis. Conventional treatments of psoriasis have adverse events that influence patients' adherence. Wannachawee Recipe (WCR) is Thai traditional medicine that is known to be effective for psoriasis patients; however, preclinical evidence is still lacking. This study investigated the therapeutic potential of WCR on antiproliferant activity using imiquimod- (IMQ-) induced psoriasis-like dermatitis in a mouse model. Psoriasis-like dermatitis was induced on the shaved dorsal skin and right ear pinna of BALB/c mice by topical application of IMQ for 15 consecutive days after which WCR was administered to the mice by oral gavage for 10 days. Phenotypical observations, histopathological examinations, and ELISA of skin and blood samples were conducted. WCR significantly ameliorated development of IMQ-induced psoriasis-like dermatitis and reduced levels of Th17 cytokines (IL-17A, IL-22, and IL-23) in both serum and dorsal skin. Histopathological findings showed a decrease in epidermal thickness and inflammatory T-cell infiltration in the WCR-treated groups. The WCR has pharmacological actions which regulate Th17 related cytokines suggesting that it is a potential alternative therapeutic strategy for psoriasis.
银屑病是一种常见的免疫介导的慢性炎症性皮肤病，其特征为出现增厚的、伴有附着银白色鳞屑的红斑性隆起斑块。T细胞通过参与银屑病发病机制的白细胞介素-23/辅助性T细胞17（IL-23/Th17）轴被激活。银屑病的传统治疗存在不良事件，会影响患者的依从性。万那差维方（WCR）是一种泰国传统药物，已知对银屑病患者有效；然而，临床前证据仍然缺乏。本研究在小鼠模型中，使用咪喹莫特（IMQ）诱导的银屑病样皮炎，研究了WCR在抗增殖活性方面的治疗潜力。通过连续15天局部应用IMQ，在BALB/c小鼠剃毛的背部皮肤和右耳廓诱导出银屑病样皮炎，之后通过口服灌胃给小鼠施用WCR，持续10天。对皮肤和血液样本进行了表型观察、组织病理学检查和酶联免疫吸附测定（ELISA）。WCR显著改善了IMQ诱导的银屑病样皮炎的发展，并降低了血清和背部皮肤中Th17细胞因子（白细胞介素-17A、白细胞介素-22和白细胞介素-23）的水平。组织病理学结果显示，WCR治疗组的表皮厚度和炎性T细胞浸润减少。WCR具有调节Th17相关细胞因子的药理作用，表明它是银屑病潜在的替代治疗策略。
