Zhang Kai, Niu Zhi-Guang, Lv Zhiwei, Zhang Ying
School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China.
School of Marine Science and Technology, Tianjin University, Tianjin, 300350, China.
Ecotoxicology. 2017 Nov;26(9):1284-1292. doi: 10.1007/s10646-017-1853-9. Epub 2017 Sep 12.
Jingjinji area occupies important position in developing of the Chinese economy, while there exists a sharp conflict between economic growth and limited water resources in this area. To ensure the safety of water consumption of cities in Jingjinji area, we investigated the abundance of three classes of antibiotic resistance genes (ARGs) in water and sediment of six water supply reservoirs in this area. The results showed that the detection frequency of sul1, tetM and ermB were 100%. However, the content ranges of these genes were different (10 to 10/16S gene copies for sul1, 10 to 10/16S gene copies for ermB, and 10 to 10/16S gene copies for tetM). The content of ribosome protection proteins (RPP) genes were the highest in all selected tet genes. The highest abundance of ARGs in water and sediment samples was sampled from Panjiakou reservoir and Guanting reservoir, respectively. Except COD, chla and tetM, there are no significant correlation between water quality parameters and ARGs. Overall, this study provides integrated profiles of the three types of ARGs in water supply reservoirs of Jingjinji area and thus helps to re-evaluate the effects of human activities to water supply reservoirs.
京津冀地区在中国经济发展中占据重要地位，然而该地区经济增长与有限水资源之间存在尖锐冲突。为确保京津冀地区城市用水安全，我们调查了该地区六个供水水库水和沉积物中三类抗生素抗性基因（ARGs）的丰度。结果表明，sul1、tetM和ermB的检出频率均为100%。然而，这些基因的含量范围不同（sul1为10至10/16S基因拷贝，ermB为10至10/16S基因拷贝，tetM为10至10/16S基因拷贝）。核糖体保护蛋白（RPP）基因的含量在所有选定的tet基因中最高。水和沉积物样本中ARGs丰度最高的分别取自潘家口水库和官厅水库。除化学需氧量（COD）、叶绿素a（chla）和tetM外，水质参数与ARGs之间无显著相关性。总体而言，本研究提供了京津冀地区供水水库中三种类型ARGs的综合概况，从而有助于重新评估人类活动对供水水库的影响。
