一种用于标记活细胞的基因编码环丁烯探针。
A genetically encoded cyclobutene probe for labelling of live cells.
作者信息
Liu K, Enns B, Evans B, Wang N, Shang X, Sittiwong W, Dussault P H, Guo J
机构信息
Department of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, USA.
出版信息
Chem Commun (Camb). 2017 Sep 21;53(76):10604-10607. doi: 10.1039/c7cc05580c.
Abstract
We have identified an aminoacyl-tRNA synthetase/tRNA pair for the efficient and site-specific incorporation of a cyclobutene-containing amino acid into proteins in response to an amber nonsense codon. Fast and fluorescent labeling of purified proteins and intact proteins in live cells was demonstrated using the inverse electron demand Diels-Alder reaction with a tetrazine.
摘要
我们已经鉴定出一种氨酰-tRNA合成酶/tRNA对，用于响应琥珀色无义密码子，将含环丁烯的氨基酸高效且位点特异性地掺入蛋白质中。利用与四嗪的逆电子需求狄尔斯-阿尔德反应，在活细胞中对纯化蛋白和完整蛋白进行了快速且荧光标记。
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