See-Too Wah-Seng, Ee Robson, Lim Yan-Lue, Convey Peter, Pearce David A, Mohidin Taznim Begam Mohd, Yin Wai-Fong, Chan Kok Gan
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
National Antarctic Research Centre (NARC), Institute of Postgraduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Stand Genomic Sci. 2017 Sep 6;12:52. doi: 10.1186/s40793-017-0264-0. eCollection 2017.
R3.8 is a psychrotolerant bacterial strain isolated from a soil sample obtained at Rothera Point, Adelaide Island, close to the Antarctic Peninsula. Strain R3.8 was sequenced in order to help discover potential cold active enzymes with biotechnological applications. Genome analysis identified various cold adaptation genes including some coding for anti-freeze proteins and cold-shock proteins, genes involved in bioremediation of xenobiotic compounds including naphthalene, and genes with chitinolytic and -acetylglucosamine utilization properties and also plant-growth-influencing properties. In this genome report, we present a complete genome sequence of strain R3.8 and its annotation data, which will facilitate exploitation of potential novel cold-active enzymes.
R3.8是从南极半岛附近阿德莱德岛罗瑟拉角采集的土壤样本中分离出的一株耐冷细菌菌株。对菌株R3.8进行了测序，以帮助发现具有生物技术应用潜力的冷活性酶。基因组分析鉴定出了各种冷适应基因，包括一些编码抗冻蛋白和冷休克蛋白的基因、参与萘等外源化合物生物修复的基因，以及具有几丁质分解和N-乙酰葡糖胺利用特性且对植物生长有影响的基因。在本基因组报告中，我们展示了菌株R3.8的完整基因组序列及其注释数据，这将有助于开发潜在的新型冷活性酶。
