Plotnikova Elena G, Ariskina Elena V, Yastrebova Olga V, Potekhina Natalia V, Avtukh Alexander N, Tarlachkov Sergey V, Starodumova Irina P, Dorofeeva Lyubov V, Evtushenko Lyudmila I
Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, Perm, 614081, Russia.
All-Russian Collection of Microorganisms (VKM), Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, 142290, Russia.
Antonie Van Leeuwenhoek. 2024 Dec 24;118(2):40. doi: 10.1007/s10482-024-02048-7.
Four salt-tolerant and aromatics degrading strains used in this study were isolated from polluted technogenic soil on the territory of the Verkhnekamsk potash deposit (Russia). The strains were aerobic, Gram-stain-positive, non-motile, non-endospore-forming irregular rods, exhibiting a marked rod-coccus growth cycle. They contained lysine-based peptidoglycan, teichulosonic acid and poly(glycosyl phosphate) polymers in the cell walls. The major menaquinone was MK-9(H), the predominant fatty acids were saturated, anteiso- and iso-branched, and the major compounds of polar lipid profiles included phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and two glycolipids (monogalactosyldiacylglycerol and dimannosylglyceride). The strains showed the highest 16S rRNA gene sequence similarity to Arthrobacter crystallopoietes (99.6-99.9%), followed by A. mangrovi (98.3-98.5%) and A. globiformis, the type species of the genus Arthrobacter (97.6-97.8%). The values of overall genome related indices (ANI, dDDH, AAI and POCP) indicated that the target strains and A. crystallopoietes represented three species within a new genus. These species clearly differed from each other and from A. mangrovi and A. globiformis in the phenotypic traits, including the peptidoglycan type and the structures and compositions of cell wall glycopolymers. Based on the findings of this study and the previously published data, we provide the description of the new genus Crystallibacter gen. nov. with the type species Crystallibacter crystallifaciens comb. nov. (type strain, VKM Ac-1107) and two newly revealed species, Crystallibacter permensis sp. nov. (type strain, B905 = VKM Ac-2550 = LMG 33113) and Crystallibacter degradans sp. nov. (type strain, SF27 = VKM Ac-2063 = LMG 33112).
本研究中使用的4株耐盐且能降解芳烃的菌株，是从俄罗斯车里雅宾斯克钾盐矿污染的技术土壤中分离得到的。这些菌株为需氧菌，革兰氏染色阳性，不运动，不形成芽孢，呈不规则杆状，呈现明显的杆-球菌生长周期。它们的细胞壁含有基于赖氨酸的肽聚糖、磷壁酸和聚（糖基磷酸）聚合物。主要的甲基萘醌是MK-9(H)，主要脂肪酸为饱和脂肪酸、anteiso-和iso-支链脂肪酸，极性脂质谱的主要化合物包括磷脂酰甘油、二磷脂酰甘油、磷脂酰肌醇和两种糖脂（单半乳糖基二酰基甘油和二甘露糖基甘油酯）。这些菌株与结晶节杆菌（99.6-99.9%）的16S rRNA基因序列相似性最高，其次是红树林节杆菌（98.3-98.5%）和节杆菌属的模式种球形节杆菌（97.6-97.8%）。全基因组相关指数（ANI、dDDH、AAI和POCP）的值表明，目标菌株和结晶节杆菌代表了一个新属中的三个物种。这些物种在表型特征上彼此明显不同，也与红树林节杆菌和球形节杆菌不同，包括肽聚糖类型以及细胞壁糖聚合物的结构和组成。基于本研究的结果和先前发表的数据，我们描述了新属结晶杆菌属（Crystallibacter gen. nov.），其模式种为结晶产菌结晶杆菌（Crystallibacter crystallifaciens comb. nov.）（模式菌株，VKM Ac-1107）以及两个新发现的物种，永久结晶杆菌（Crystallibacter permensis sp. nov.）（模式菌株，B905 = VKM Ac-2550 = LMG 33113）和降解结晶杆菌（Crystallibacter degradans sp. nov.）（模式菌株，SF27 = VKM Ac-2063 = LMG 33112）。
