Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Kyonggi-Do, 16227, Republic of Korea.
Department of Environmental Engineering, Korea University Sejong Campus, Sejong City, 30019, Republic of Korea.
Arch Microbiol. 2021 Jan;203(1):377-382. doi: 10.1007/s00203-020-02048-x. Epub 2020 Sep 22.
A non-motile, Gram-stain-negative, rod-shaped and yellow-colored bacterium, designated G-1-1-1 was obtained from soil sampled at Gwanggyo stream bank, Gyeonggi-do, Republic of Korea. Cells were aerobic, catalase positive, grew optimally at 25-30 °C and hydrolysed aesculin and casein. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain G-1-1-1 formed a lineage within the genus Luteolibacter. The closest members were Luteolibacter flavescens GKX (97.7% sequence similarity) and Luteolibacter arcticus MC 3726 (97.3%). The sequence similarities with other members of the genus Luteolibacter were ≤ 93.9%. The genome of strain G-1-1-1 was 6,412,079 bp long with 5176 protein-coding genes. The diagnostic amino acid of cell-wall peptidoglycan of strain G-1-1-1 was meso-diaminopimelic acid. The only respiratory quinone was menaquinone-9 and the principal polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and unidentified phospholipids. The predominant cellular fatty acids were iso-C, C ω9c, C, C and anteiso-C. The DNA G + C content was 61.0 mol%. The anti-SMASH analysis of whole genome showed eight putative biosynthetic gene clusters responsible for various secondary metabolites. Based on genomic, chemotaxonomic, phenotypic and phylogenetic analyses, strain G-1-1-1 represents a novel species in the genus Luteobacter, for which the name Luteolibacter luteus sp. nov. is proposed. The type strain is G-1-1-1 (= KACC 21614 = NBRC 114341).
一株非运动、革兰氏阴性、杆状、黄色的细菌，被命名为 G-1-1-1，是从韩国京畿道光州溪岸的土壤中分离得到的。细胞为需氧型，过氧化氢酶阳性，最适生长温度为 25-30°C，可水解马栗树皮素和酪蛋白。基于 16S rRNA 基因序列的系统发育分析表明，菌株 G-1-1-1 形成了黄杆菌属内的一个谱系。最接近的成员是黄杆菌属的 Flavescens GKX（97.7%序列相似性）和北极黄杆菌 MC 3726（97.3%）。与该属其他成员的序列相似度均≤93.9%。菌株 G-1-1-1 的基因组长 6,412,079bp，包含 5176 个蛋白编码基因。细胞壁肽聚糖的特征氨基酸为meso-二氨基庚二酸。唯一的呼吸醌是menaquinone-9，主要极性脂是磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰甘油和未鉴定的磷脂。主要的细胞脂肪酸是 iso-C、C ω9c、C 14:0、C 16:0 和 anteiso-C。DNA G+C 含量为 61.0mol%。全基因组的 anti-SMASH 分析显示了八个负责各种次生代谢产物的假定生物合成基因簇。基于基因组、化学分类学、表型和系统发育分析，菌株 G-1-1-1 代表黄杆菌属的一个新种，命名为黄杆菌 luteus sp. nov.。模式菌株为 G-1-1-1（=KACC 21614=NBRC 114341）。
