Pascual Javier, Foesel Bärbel U, Geppert Alicia, Huber Katharina J, Boedeker Christian, Luckner Manja, Wanner Gerhard, Overmann Jörg
Department of Microbial Ecology and Diversity Research, Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.
Present address: Research Unit Comparative Microbiome Analysis, Helmholtz Zentrum München - German Research Center for Environmental Health (GmbH), Neuherberg, Germany.
Int J Syst Evol Microbiol. 2018 Apr;68(4):1028-1036. doi: 10.1099/ijsem.0.002619. Epub 2018 Feb 14.
A novel slow-growing bacterium, designated strain AW1220, was isolated from agricultural floodplain soil sampled at Mashare (Kavango region, Namibia) by using a high-throughput cultivation approach. Strain AW1220 was characterized as a Gram-negative, non-motile, rod-shaped bacterium. Occasionally, some cells attained an unusual length of up to 35 µm. The strain showed positive responses for catalase and cytochrome-c oxidase and divided by binary fission and/or budding. The strain had an aerobic chemoorganoheterotrophic metabolism and was also able to grow under micro-oxic conditions. Colonies were small and pink pigmented. Strain AW1220 was found to be a mesophilic, neutrophilic and non-halophilic bacterium. Cells accumulated polyphosphate intracellularly and mainly utilized complex protein substrates for growth. 16S rRNA gene sequence comparisons revealed that strain AW1220 belonged to the class Gemmatimonadetes (=group 1). Its closest relatives were found to be Gemmatimonas aurantiaca T-27 (90.9 % gene sequence similarity), Gemmatimonas phototrophica AP64 (90.8 %) and Longimicrobiumterrae CB-286315 (84.2 %). The genomic G+C content was 73.3 mol%. The major fatty acids were iso-C15 : 0, C16 : 1ω7c and/or iso-C15 : 0 2-OH, iso-C17 : 1ω9c, iso-C15 : 0 3-OH and C16 : 0. The predominant respiratory quinone was MK-9, albeit minor amounts of MK-8 and MK-10 are also present. The polar lipids comprised major amounts of phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol and one unidentified phosphoglycolipid. On the basis of its polyphasic characterization, strain AW1220 represents a novel genus and species of the class Gemmatimonadetes for which the name Roseisolibacter agri gen. nov., sp. nov. is proposed, with the type strain AW1220 (=DSM 104292=LMG 29977).
通过高通量培养方法，从纳米比亚卡万戈地区马沙雷采集的农业洪泛区土壤中分离出一种新型的生长缓慢的细菌，命名为AW1220菌株。AW1220菌株被鉴定为革兰氏阴性、无运动性、杆状细菌。偶尔，一些细胞会达到长达35 µm的异常长度。该菌株过氧化氢酶和细胞色素c氧化酶反应呈阳性，通过二分裂和/或出芽进行分裂。该菌株具有需氧化学有机异养代谢，也能够在微氧条件下生长。菌落小且呈粉红色。发现AW1220菌株是嗜温、嗜中性和非嗜盐细菌。细胞在细胞内积累多磷酸盐，主要利用复杂蛋白质底物进行生长。16S rRNA基因序列比较表明，AW1220菌株属于芽单胞菌纲（=第1组）。发现其最接近的亲缘种是橙色芽单胞菌T-27（基因序列相似性为90.9%）、光合芽单胞菌AP64（90.8%）和土壤长微菌CB-286315（84.2%）。基因组G+C含量为73.3 mol%。主要脂肪酸为异C15:0、C16:1ω7c和/或异C15:0 2-OH、异C17:1ω9c、异C15:0 3-OH和C16:0。主要呼吸醌为MK-9，不过也存在少量的MK-8和MK-10。极性脂质主要包括磷脂酰乙醇胺、磷脂酰胆碱、二磷脂酰甘油和一种未鉴定的磷酸糖脂。基于其多相特征，AW1220菌株代表芽单胞菌纲中的一个新属和新种，为此提出新属名为玫瑰土壤杆菌属（Roseisolibacter），新种名为玫瑰土壤杆菌（Roseisolibacter agri），模式菌株为AW1220（=DSM 104292=LMG 29977）。
