Perez Gutierrez R M, Lule P R
a Laboratorio de Investigacion de Productos Naturales, Escuela Superior de Ingenieria Quimica e Industias extractivas IPN, Col. Torres Lindavista, Mexico D.F., Mexico.
Pharm Biol. 2005;43(4):313-316. doi: 10.1080/13880200590951702.
Activity-guided fractionation of the methanol extract of Daphnia pulex. (Leydig) using the MTT assay and P-388, A549, KB, and HT-29 cell lines permitted the isolation of four known glyceroglycolipid compounds: (2S.)-1-O.-(7Z.,10Z.,13Z.)-hexadecatrienoyl-2-O.-(7Z.,10Z.)-hexadecadienoyl-3-O.-β.-D-galactopyranosyl-sn.-glycerol, (2S.)-1-O.-(7Z.,10Z.)-hexadecadienoyl-2-O.-(7Z.,10Z.)-hexadecadienoyl-3-O.-β.-D-galactopyranosyl-sn.-glycerol, (2S.)-1-O.-(7Z.,10Z.)-hexadecadienolyl-2-O.-linolenoyl-3-O.-β.-D-galactopyranosyl-sn.-glycerol, and (2S.)-1-O.-(9Z., 12Z.)-octadecadienoyl-2-O.-(7Z.,10Z.)-hexadecadienoyl-3-O.-(α.-D-galactopyranosyl(1-6)-β.-D-galactopyranosyl-sn.-glycerol. All the isolates were found to be moderately cytotoxic (ED < 4 µg/ml) toward P-388, A549, KB, and HT-29 cell lines.
采用MTT法以及P - 388、A549、KB和HT - 29细胞系，对蚤状溞（Leydig）甲醇提取物进行活性导向分级分离，从中分离出四种已知的甘油糖脂化合物：(2S.)-1-O.-(7Z.,10Z.,13Z.)-十六碳三烯酰基-2-O.-(7Z.,10Z.)-十六碳二烯酰基-3-O.-β.-D-吡喃半乳糖基-sn.-甘油、(2S.)-1-O.-(7Z.,10Z.)-十六碳二烯酰基-2-O.-(7Z.,10Z.)-十六碳二烯酰基-3-O.-β.-D-吡喃半乳糖基-sn.-甘油、(2S.)-1-O.-(7Z.,10Z.)-十六碳二烯醇基-2-O.-亚麻酸酰基-3-O.-β.-D-吡喃半乳糖基-sn.-甘油，以及(2S.)-1-O.-(9Z.,12Z.)-十八碳二烯酰基-2-O.-(7Z.,10Z.)-十六碳二烯酰基-3-O.-(α.-D-吡喃半乳糖基(1-6)-β.-D-吡喃半乳糖基-sn.-甘油。结果发现，所有分离物对P - 388、A549、KB和HT - 29细胞系均具有中等细胞毒性（ED < 4 µg/ml）。
