Liu Dongqin, Wang Ying, Lu Zhaoxin, Lv Fengxia, Bie Xiaomei, Zhao Haizhen
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.
Nat Prod Res. 2023 Oct-Nov;37(21):3610-3615. doi: 10.1080/14786419.2022.2095637. Epub 2022 Jul 6.
The study was to optimize the separation procedures, characterize the galactoglycerolipids and explore their anti-inflammatory activities. Two monogalactosyldiacylglycerols (MGDGs) and three digalactosyldiacylglycerols (DGDGs) from (L.) Britton were obtained through one-step silica gel column chromatography and preparative high-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD). The presence of additional MGDG (1--9,12,15-octadecatrienoyl-2--7,10,13-hexadecatrienoyl-3--(-D-galactopyranosyl)--glycerol) and DGDG (1--9,12-octadecadienoyl-2--9,12,15-octadecatrienoyl-3--(-D-galactopyranosyl-(1'→6'')--D-galactopyranosyl)--glycerol) was concluded for the first time in perilla, by liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR). In lipopolysaccharide (LPS)-induced RAW264.7 cells, five galactoglycerolipids exhibited good inhibitory activities against nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) gene expression in a dose-dependent manner, suggesting that fatty acid chain length and unsaturation degree affected their anti-inflammatory activities.
本研究旨在优化分离程序，表征半乳糖甘油酯并探索其抗炎活性。通过一步硅胶柱色谱法和配有蒸发光散射检测器的制备型高效液相色谱法（HPLC-ELSD），从紫苏（L.）布里顿中获得了两种单半乳糖基二酰基甘油（MGDG）和三种双半乳糖基二酰基甘油（DGDG）。通过液相色谱-质谱联用（LC-MS）和核磁共振（NMR）首次在紫苏中鉴定出另外的MGDG（1--9,12,15-十八碳三烯酰基-2--7,10,13-十六碳三烯酰基-3--(-D-吡喃半乳糖基)--甘油）和DGDG（1--9,12-十八碳二烯酰基-2--9,12,15-十八碳三烯酰基-3--(-D-吡喃半乳糖基-(1'→6'')--D-吡喃半乳糖基)--甘油）。在脂多糖（LPS）诱导的RAW264.7细胞中，五种半乳糖甘油酯对一氧化氮（NO）生成和诱导型一氧化氮合酶（iNOS）基因表达表现出良好的剂量依赖性抑制活性，表明脂肪酸链长度和不饱和度影响它们的抗炎活性。
