Departments of Electrical and Computer Engineering, Boston University, Boston, MA, 02215, United States.
The Photonics Center, Boston University, Boston, MA, 02215, United States.
Sci Rep. 2017 Sep 20;7(1):11951. doi: 10.1038/s41598-017-12377-5.
Femtosecond (fs) pulsed laser irradiation techniques have attracted interest as a photonic approach for the selective inactivation of virus contaminations in biological samples. Conventional pulsed laser approaches require, however, relatively long irradiation times to achieve a significant inactivation of virus. In this study, we investigate the enhancement of the photonic inactivation of Murine Leukemia Virus (MLV) via 805 nm femtosecond pulses through gold nanorods whose localized surface plasmon resonance overlaps with the excitation laser. We report a plasmonically enhanced virus inactivation, with greater than 3.7-log reduction measured by virus infectivity assays. Reliable virus inactivation was obtained for 10 s laser exposure with incident laser powers ≥0.3 W. Importantly, the fs-pulse induced inactivation was selective to the virus and did not induce any measurable damage to co-incubated antibodies. The loss in viral infection was associated with reduced viral fusion, linking the loss in infectivity with a perturbation of the viral envelope. Based on the observations that physical contact between nanorods and virus particles was not required for viral inactivation and that reactive oxygen species (ROS) did not participate in the detected viral inactivation, a model of virus inactivation based on plasmon enhanced shockwave generation is proposed.
飞秒（fs）脉冲激光辐照技术作为一种用于生物样品中病毒污染选择性灭活的光子方法引起了人们的兴趣。然而，传统的脉冲激光方法需要相对较长的辐照时间才能实现病毒的显著灭活。在这项研究中，我们通过金纳米棒研究了通过与激发激光重叠的 805nm 飞秒脉冲增强对鼠白血病病毒（MLV）的光灭活作用。我们报告了一种等离子体增强的病毒灭活作用，通过病毒感染性测定测量得到大于 3.7 对数的减少。对于 10 秒激光暴露，使用≥0.3W 的入射激光功率可获得可靠的病毒灭活作用。重要的是，fs 脉冲诱导的失活作用对病毒具有选择性，并且不会对共孵育的抗体产生任何可测量的损伤。病毒感染的丧失与病毒融合减少有关，将感染性丧失与病毒包膜的扰动联系起来。基于以下观察结果，即纳米棒与病毒颗粒之间的物理接触不是病毒失活所必需的，并且活性氧（ROS）不参与检测到的病毒失活，提出了一种基于等离子体增强冲击波产生的病毒失活模型。
