Department of Agricultural and Food Science, Faculty of Science, Universiti Tunku Abdul Rahman, Jalan Universiti, Bandar Barat, 31900 Kampar, Perak, Malaysia.
Laboratory for Intestinal Ecosystem, RIKEN Center for Integrative Medical Sciences, Kanagawa 230-0045, Japan; Immunobiology Laboratory, Graduate School of Medical Life Science, Yokohama City University, Kanawaga 230-0045, Japan.
J Biotechnol. 2017 Nov 20;262:75-83. doi: 10.1016/j.jbiotec.2017.09.007. Epub 2017 Sep 19.
In this study, hypercholesterolemic mice fed with Lactobacillus fermentum FTDC 8312 after a seven-week feeding trial showed a reduction in serum total cholesterol (TC) levels, accompanied by a decrease in serum low-density lipoprotein cholesterol (LDL-C) levels, an increase in serum high-density lipoprotein cholesterol (HDL-C) levels, and a decreased ratio of apoB100:apoA1 when compared to those fed with control or a type strain, L. fermentum JCM 1173. These have contributed to a decrease in atherogenic indices (TC/HDL-C) of mice on the FTDC 8312 diet. Serum triglyceride (TG) levels of mice fed with FTDC 8312 and JCM 1173 were comparable to those of the controls. A decreased ratio of cholesterol and phospholipids (C/P) was also observed for mice fed with FTDC 8312, leading to a decreased number of spur red blood cells (RBC) formation in mice. Additionally, there was an increase in fecal TC, TG, and total bile acid levels in mice on FTDC 8312 diet compared to those with JCM 1173 and controls. The administration of FTDC 8312 also altered the gut microbiota population such as an increase in the members of genera Akkermansia and Oscillospira, affecting lipid metabolism and fecal bile excretion in the mice. Overall, we demonstrated that FTDC 8312 exerted a cholesterol lowering effect that may be attributed to gut microbiota modulation.
在这项研究中，经过七周的喂养试验，喂食嗜酸乳杆菌 FTDC8312 的高胆固醇血症小鼠的血清总胆固醇（TC）水平降低，同时血清低密度脂蛋白胆固醇（LDL-C）水平降低，血清高密度脂蛋白胆固醇（HDL-C）水平升高，载脂蛋白 B100:载脂蛋白 A1 比值降低。与对照组或对照菌株嗜酸乳杆菌 JCM1173 相比，这些变化导致了 FTDC8312 饮食的小鼠动脉粥样硬化指数（TC/HDL-C）降低。FTDC8312 和 JCM1173 喂养的小鼠血清甘油三酯（TG）水平与对照组相当。FTDC8312 喂养的小鼠胆固醇与磷脂（C/P）比值也降低，导致小鼠红细胞（RBC）形成的刺状红细胞减少。此外，与 JCM1173 和对照组相比，FTDC8312 饮食的小鼠粪便 TC、TG 和总胆汁酸水平增加。FTDC8312 的给药还改变了肠道微生物群的组成，例如阿克曼氏菌属和 Oscillospira 属成员的增加，影响了小鼠的脂质代谢和粪便胆汁排泄。总的来说，我们证明了 FTDC8312 具有降低胆固醇的作用，这可能归因于肠道微生物群的调节。
