Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Department of Clinical Study, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Probiotics Antimicrob Proteins. 2019 Dec;11(4):1163-1171. doi: 10.1007/s12602-018-9480-1.
The aim of this study was to investigate the effect of Lactobacillus plantarum and Bacillus coagulans on serum lipid profile and lowering potential of probiotic in hypercholesterolemic rats. Twenty-eight male Wistar rats were divided into four groups as follows: (1) control group, fed standard commercial diet; (2) HC group, fed high-cholesterol diet; (3) HC + LP group, fed high-cholesterol diet and gavaging of L. plantarum; and (4) HC + BC group fed high-cholesterol diet and gavaging of B. coagulans. After 28 and 50 days, serum lipid profile; serum ALT and AST; the body and organ weights; fecal total count; Enterobacteriaceae, L. plantarum, and B. coagulans counts; and blood glucose tolerance were measured. We observed that levels of triglyceride, cholesterol, LDL, VLDL, and atherogenic index in serum were significantly lower in the HC + probiotic groups. Also, serum ALT and AST were significantly decreased in probiotic-treated groups. In addition, we found that feeding of a high-cholesterol diet for 50 days produced significant increases in the body weight, in addition to the fact that the administration of L. plantarum and B. coagulans has considerably reduced the body weight gain. B. coagulans and L. plantarum can survive passing through the upper-gastrointestinal tract after oral feeding to the rats and colonized in their colon. These bacteria could be exploited as a potential biotherapeutic remedy to reduce TC, TG, LDL, VLDL, and atherogenic index in hypercholesterolemic condition.
本研究旨在探讨植物乳杆菌和凝结芽孢杆菌对血清脂质谱的影响及其在高胆固醇血症大鼠中的潜在降血脂作用。将 28 只雄性 Wistar 大鼠分为四组：（1）对照组，喂食标准商业饮食；（2）HC 组，喂食高胆固醇饮食；（3）HC+LP 组，喂食高胆固醇饮食并灌胃植物乳杆菌；（4）HC+BC 组，喂食高胆固醇饮食并灌胃凝结芽孢杆菌。在第 28 天和第 50 天测量血清脂质谱、血清 ALT 和 AST、体重和器官重量、粪便总计数、肠杆菌科、植物乳杆菌和凝结芽孢杆菌计数以及血糖耐量。结果显示，HC+益生菌组血清中甘油三酯、胆固醇、LDL、VLDL 和致动脉粥样硬化指数水平显著降低。此外，益生菌治疗组血清 ALT 和 AST 显著降低。此外，我们发现，50 天高胆固醇饮食喂养使体重显著增加，而植物乳杆菌和凝结芽孢杆菌的给药可显著降低体重增加。植物乳杆菌和凝结芽孢杆菌可以在口服给予大鼠后通过上消化道存活并在其结肠中定植。这些细菌可以作为一种潜在的生物治疗方法，用于降低高胆固醇血症患者的 TC、TG、LDL、VLDL 和致动脉粥样硬化指数。
