幽门螺杆菌α1-3/4-岩藻糖基转移酶（Hp3/4FT）催化的Lewis抗原和人乳岩藻糖苷的一锅多酶（OPME）合成。
H. pylori α1-3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides.
作者信息
Yu Hai, Li Yanhong, Wu Zhigang, Li Lei, Zeng Jie, Zhao Chao, Wu Yijing, Tasnima Nova, Wang Jing, Liu Huaide, Gadi Madhusudhan Reddy, Guan Wanyi, Wang Peng G, Chen Xi
机构信息
Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA.
出版信息
Chem Commun (Camb). 2017 Oct 5;53(80):11012-11015. doi: 10.1039/c7cc05403c.
Abstract
Helicobacter pylori α1-3/4-fucosyltransferase (Hp3/4FT) was expressed in Escherichia coli at a level of 30 mg L culture and used as a diverse catalyst in a one-pot multienzyme (OPME) system for high-yield production of l-fucose-containing carbohydrates including Lewis antigens such as Lewis a, b, and x, O-sulfated Lewis x, and sialyl Lewis x and human milk fucosides such as 3-fucosyllactose (3-FL), lacto-N-fucopentaose (LNFP) III, and lacto-N-difuco-hexaose (LNDFH) II and III. Noticeably, while difucosylation of tetrasaccharides was readily achieved using an excess amount of donor, the synthesis of LNFP III was achieved by Hp3/4FT-catalyzed selective fucosylation of the N-acetyllactosamine (LacNAc) component in lacto-N-neotetraose (LNnT).
摘要
幽门螺杆菌α1-3/4-岩藻糖基转移酶（Hp3/4FT）在大肠杆菌中表达，产量为每升培养物30毫克，并在一锅多酶（OPME）系统中用作多种催化剂，用于高产生产含L-岩藻糖的碳水化合物，包括Lewis抗原，如Lewis a、b和x、O-硫酸化Lewis x和唾液酸化Lewis x，以及人乳岩藻糖苷，如3-岩藻糖基乳糖（3-FL）、乳糖-N-岩藻五糖（LNFP）III和乳糖-N-二岩藻六糖（LNDFH）II和III。值得注意的是，虽然使用过量供体很容易实现四糖的双岩藻糖基化，但LNFP III的合成是通过Hp3/4FT催化乳糖-N-新四糖（LNnT）中N-乙酰乳糖胺（LacNAc）成分的选择性岩藻糖基化实现的。
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