Frank D W, Parker C D
J Biol Stand. 1984 Oct;12(4):353-65. doi: 10.1016/s0092-1157(84)80060-8.
Monoclonal antibodies to Bordetella pertussis were produced by fusion of mouse myeloma cells and spleen cells of immunized mice. Cell lines were examined for specific antibody production against several crude antigen preparations and lipopolysaccharide. Cross reactivity of monoclonal antibodies was assessed by enzyme immunoassay using cell lysates prepared from Bordetella spp. and several other bacteria. Reactivity of monoclonal antibodies to cell surface components was determined by immunofluorescence microscopy. Monoclonal antibodies represent useful probes to study the antigenic profile and distribution of antigens among various species of Bordetella, as well as specific tools to study the structure and function of B. pertussis virulence factors.
通过将小鼠骨髓瘤细胞与免疫小鼠的脾细胞融合，制备了针对百日咳博德特氏菌的单克隆抗体。检测细胞系针对几种粗抗原制剂和脂多糖产生特异性抗体的情况。使用从博德特氏菌属和其他几种细菌制备的细胞裂解物，通过酶免疫测定法评估单克隆抗体的交叉反应性。通过免疫荧光显微镜确定单克隆抗体对细胞表面成分的反应性。单克隆抗体是研究博德特氏菌不同物种间抗原谱和抗原分布的有用探针，也是研究百日咳博德特氏菌毒力因子结构和功能的特异性工具。
