Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 Taibai North Road, Xi'an, Shaanxi Province, 710069, People's Republic of China.
Shaanxi Institute of Zoology, 88 Xingqing Road, Xi'an, Shaanxi Province, 710032, People's Republic of China.
BMC Genomics. 2017 Sep 22;18(1):750. doi: 10.1186/s12864-017-4125-4.
Salamanders regenerate their limbs after amputation. However, the molecular mechanism of this unique regeneration remains unclear. In this study, isobaric tags for relative and absolute quantification (iTRAQ) coupled with liquid chromatography tandem mass spectrometry (LC-MS/MS) was employed to quantitatively identify differentially expressed proteins in regenerating limbs 3, 7, 14, 30 and 42 days post amputation (dpa).
Of 2636 proteins detected in total, 253 proteins were differentially expressed during different regeneration stages. Among these proteins, Asporin, Cadherin-13, Keratin, Collagen alpha-1(XI) and Titin were down-regulated. CAPG, Coronin-1A, AnnexinA1, Cathepsin B were up-regulated compared with the control. The identified proteins were further analyzed to obtain information about their expression patterns and functions in limb regeneration. Functional analysis indicated that the differentially expressed proteins were associated with wound healing, immune response, cellular process, metabolism and binding.
This work indicated that significant proteome alternations occurred during salamander limb regeneration. The results may provide fundamental knowledge to understand the mechanism of limb regeneration.
蝾螈在肢体截肢后会再生。然而，这种独特再生的分子机制尚不清楚。在这项研究中，采用相对和绝对定量同位素标签（iTRAQ）联合液相色谱串联质谱（LC-MS/MS）技术定量鉴定了截肢后 3、7、14、30 和 42 天再生肢体中的差异表达蛋白。
在总共检测到的 2636 种蛋白质中，有 253 种在不同的再生阶段表达差异。在这些蛋白质中，Asprins、Cadherin-13、角蛋白、胶原 alpha-1(XI) 和 Titin 下调。与对照组相比，CAPG、Coronin-1A、AnnexinA1 和组织蛋白酶 B 上调。鉴定出的蛋白质进一步进行了分析，以获得有关其在肢体再生中表达模式和功能的信息。功能分析表明，差异表达的蛋白质与伤口愈合、免疫反应、细胞过程、代谢和结合有关。
这项工作表明，蝾螈肢体再生过程中发生了显著的蛋白质组改变。研究结果可能为理解肢体再生机制提供基础知识。
