Biological Materials Laboratory, CSIR-Central Leather Research Institute, Chennai 600020, India.
Biomed Mater. 2017 Sep 25;12(5):055008. doi: 10.1088/1748-605X/aa7d5a.
Polymers from renewable resources are attractive for various industrial and biomedical applications owing to their compatibility, degradability, ease of use and availability. Rapid progress in the development of nanotechnology has improved the characteristic features of polymers in composite materials by reinforcing the nanosized particulates during fabrication. In this study, we have attempted to incorporate metal oxide nanoparticles into polymeric nanofibers in order to enhance the overall properties of the composite scaffold. The thermal stability of a TiO nanoparticle-impregnated zein-polydopamine-based nanofibrous scaffold was investigated, and its potential as a suitable wound dressing material was demonstrated. Further, the influence of nanotopographic structure on improved adhesion, proliferation and migration of cells was ascertained through in vitro assays. The constructive results obtained were well corroborated with the in vivo excisional wound healing experiment. Thus, the competence of the prepared nanofibrous scaffold was examined both in vitro and in vivo and demonstrated to be an alternative, cost-effective biomaterial for skin tissue engineering applications.
由于可再生资源聚合物的相容性、可降解性、易用性和可用性，它们在各个工业和生物医学领域的应用中具有吸引力。纳米技术的快速发展通过在制造过程中增强纳米颗粒来改善复合材料中聚合物的特性。在这项研究中，我们试图将金属氧化物纳米粒子纳入聚合物纳米纤维中，以提高复合材料支架的整体性能。研究了 TiO2 纳米颗粒浸渍的玉米醇溶蛋白-聚多巴胺基纳米纤维支架的热稳定性，并证明了其作为合适的伤口敷贴材料的潜力。此外，通过体外实验确定了纳米形貌结构对细胞黏附、增殖和迁移的影响。通过体内实验验证了所获得的构建结果，结果与体内实验吻合良好。因此，对制备的纳米纤维支架进行了体外和体内测试，证明它是一种用于皮肤组织工程应用的具有成本效益的替代生物材料。
