Ru Jiangying, Wei Qiang, Yang Lianqing, Qin Jing, Tang Liangchen, Wei Jie, Guo Lieping, Niu Yunfei
Department of Orthopaedics, The Affiliated Hospital of Yangzhou University, Yangzhou University Yangzhou 225009 China.
Department of Orthopaedics, Changhai Hospital, Second Military Medical University Shanghai 200433 China
RSC Adv. 2018 May 22;8(34):18745-18756. doi: 10.1039/c8ra02595a.
Bioactive and degradable scaffolds of nano magnesium silicate (n-MS)/zein (ZN)/poly(caprolactone) (PCL) ternary composites were prepared by 3D-printing method. The results showed that the 3D-printed scaffolds possessed controllable pore structure, and pore morphology, pore size, porosity and pore interconnectivity of the scaffolds can be efficiently adjusted. In addition, the apatite-mineralization ability of the scaffolds in simulated body fluids was obviously improved with the increase of ZN content, in which the scaffold with 20 w% ZN (C20) possessed excellent apatite-mineralization ability. Moreover, the degradability of the scaffolds was significantly enhanced with the increase of ZN content in the scaffolds. The degradation of ZN produced acidic products that could neutralize the alkaline products from the degradation of n-MS, which avoid the increase of pH value in degradable solution. Furthermore, the MC3T3-E1 cells responses ( proliferation and differentiation, ) to the scaffolds were significantly promoted with the increase of ZN content. The osteogenesis of the scaffolds implanted the femur defects of rabbits was investigated by micro-CT and histological analysis. The results demonstrated that the new bone formation was significantly enhanced with the increase of ZN content, in which the C20 scaffold induced the highest new bone tissues, indicating excellent osteogenesis. The results suggested that the ZN in the ternary composite scaffolds played key roles in assisting bone regeneration .
采用3D打印方法制备了纳米硅酸镁（n-MS）/玉米醇溶蛋白（ZN）/聚己内酯（PCL）三元复合材料的生物活性可降解支架。结果表明，3D打印支架具有可控的孔结构，其孔形态、孔径、孔隙率和孔连通性均可有效调节。此外，随着ZN含量的增加，支架在模拟体液中的磷灰石矿化能力明显提高，其中含20 w% ZN的支架（C20）具有优异的磷灰石矿化能力。而且，随着支架中ZN含量的增加，支架的降解性显著增强。ZN的降解产生酸性产物，可中和n-MS降解产生的碱性产物，避免降解溶液pH值升高。此外，随着ZN含量的增加，MC3T3-E1细胞对支架的反应（增殖和分化）显著增强。通过显微CT和组织学分析研究了植入兔股骨缺损处的支架的成骨情况。结果表明，随着ZN含量的增加，新骨形成显著增强，其中C20支架诱导的新骨组织最多，表明其具有优异的成骨能力。结果表明，三元复合支架中的ZN在辅助骨再生中起关键作用。
