1 Department of Oral Anatomy, School of Dentistry, Pusan National University, Yangsan, South Korea.
2 School of Electrical and Computer Engineering, Pusan National University, Busan, South Korea.
J Dent Res. 2018 Feb;97(2):179-183. doi: 10.1177/0022034517733958. Epub 2017 Sep 25.
This study aimed to apply fluoride formulations to enamel with cold atmospheric plasma (CAP) and analyze the fluoride uptake, retention, and acid resistance quantitatively. Human enamel specimens were divided randomly into 2 groups: group APF1, 1.23% acidulated phosphate fluoride (APF) gel; group APF2, 1.23% APF gel with CAP. Fluoride and CAP were applied to the samples 4 times at 1-wk intervals. The specimens were also stored in artificial saliva for 4 wk to evaluate the retention of fluoride. The fluoride content on the fluoride-treated enamel was measured by an electron probe microanalyzer. To detect the resistance to demineralization, the calcium-to-phosphate ratio of the enamel samples was measured after the application of APF gel with or without CAP, followed by soaking in the demineralization solution. In groups APF1 and APF2, the amount of fluoride detected increased depending on the application frequency, and more fluoride was detected in group APF2 than in group APF1. In the experiment examining the maintenance effect, fluoride was not detected in group APF1, whereas fluoride was detected in group APF2 up to the fourth week. As for the resistance to demineralization, the calcium-to-phosphate ratio of the enamel treated with APF and CAP was higher than that treated with APF alone, and it increased with the frequency of treatment. This study suggests that the combination treatment of CAP and fluoride improves retention of fluoride on the enamel and resistance to demineralization when compared with treatment with fluoride alone.
本研究旨在应用冷等离子体(CAP)将氟化物配方施用到牙釉质上,并定量分析氟化物的摄取、保留和抗酸性。将人类牙釉质标本随机分为 2 组:APF1 组,1.23%酸性磷酸氟化物(APF)凝胶;APF2 组,含 CAP 的 1.23%APF 凝胶。在 1 周的间隔内,4 次将氟化物和 CAP 应用于样本。将标本还储存在人工唾液中 4 周,以评估氟化物的保留情况。用电子探针微分析仪测量氟化物处理牙釉质上的氟化物含量。为了检测抗脱矿能力,在用或不用 CAP 施加 APF 凝胶后,测量牙釉质样本的钙-磷比,然后将其浸泡在脱矿溶液中。在 APF1 和 APF2 组中,随着应用频率的增加,检测到的氟化物量增加,并且在 APF2 组中检测到的氟化物量多于 APF1 组。在检查维持效果的实验中,APF1 组中未检测到氟化物,而 APF2 组中直至第 4 周仍检测到氟化物。就抗脱矿性而言,用 APF 和 CAP 处理的牙釉质的钙-磷比高于单独用 APF 处理的牙釉质,并且随着处理频率的增加而增加。本研究表明,与单独用氟化物处理相比,CAP 和氟化物的联合处理可提高牙釉质上氟化物的保留和抗脱矿能力。