Christoph J, Schröder-Schetelig J, Luther S
Max Planck Institute for Dynamics and Self-Organization, Am Fassberg 17, 37077 Göttingen, Germany; German Center for Cardiovascular Research, Partner Site Göttingen, Germany.
Max Planck Institute for Dynamics and Self-Organization, Am Fassberg 17, 37077 Göttingen, Germany; University of Goettingen, Institute for Nonlinear Dynamics, Göttingen, Germany.
Prog Biophys Mol Biol. 2017 Nov;130(Pt B):150-169. doi: 10.1016/j.pbiomolbio.2017.09.015. Epub 2017 Sep 22.
Optical mapping is a widely used imaging technique for investigating cardiac electrophysiology in intact, Langendorff-perfused hearts. Mechanical contraction of cardiac tissue, however, may result in severe motion artifacts and significant distortion of the fluorescence signals. Therefore, pharmacological uncoupling is widely used to reduce tissue motion. Recently, various image processing algorithms have been proposed to reduce motion artifacts. We will review these technological developments. Furthermore, we will present a novel approach for the three-dimensional, marker-free reconstruction of contracting Langendorff-perfused intact hearts under physiological conditions. The algorithm allows disentangling the fluorescence signals (e.g. membrane voltage or intracellular calcium) from the mechanical motion (e.g. tissue strain). We will discuss the algorithms reconstruction accuracy, resolution, and robustness using experimental data from Langendorff-perfused rabbit hearts.
光学映射是一种广泛应用的成像技术，用于研究完整的、Langendorff灌注心脏的心脏电生理学。然而，心脏组织的机械收缩可能会导致严重的运动伪影和荧光信号的显著失真。因此，药理学解偶联被广泛用于减少组织运动。最近，人们提出了各种图像处理算法来减少运动伪影。我们将回顾这些技术发展。此外，我们将提出一种新颖的方法，用于在生理条件下对收缩的Langendorff灌注完整心脏进行三维、无标记重建。该算法允许将荧光信号（如膜电压或细胞内钙）与机械运动（如组织应变）分离。我们将使用Langendorff灌注兔心脏的实验数据来讨论算法的重建准确性、分辨率和鲁棒性。
