Ohtsu T, Tobinai K, Minato K, Mukai K, Kagami Y, Miwa M, Arai C, Shimoyama M
Department of Internal Medicine, National Cancer Center Hospital, Tokyo.
Jpn J Clin Oncol. 1988 Mar;18(1):33-41. doi: 10.1093/jjco/18.1.33.
A 49-year-old man developed adult T-cell leukemia (ATL) and acute myeloblastic leukemia (AML) at the same time. Using Southern blotting analysis, the leukemic cells of the ATL were found to contain the human T-cell leukemia virus type I (HTLV-I) proviral genome, whereas those of the AML did not, indicating the HTLV-I not to be associated with the AML oncogenesis. At the initial presentation, the serum anti-HTLV-I antibody was judged on screening by a routine particle-agglutination (PA) test and an indirect immunofluorescence assay (IF) to be negative. By Western blotting analysis, however, the serum was proved to be positive for anti-HTLV-I antibody. These results indicate that a routine PA-test and an IF may show false negative reactions on very rare occasions of low antibody titer. This is the first report of a coincidence of ATL with another type of leukemia.
一名49岁男性同时患上成人T细胞白血病（ATL）和急性髓细胞白血病（AML）。通过Southern印迹分析发现，ATL的白血病细胞含有I型人类T细胞白血病病毒（HTLV-I）前病毒基因组，而AML的白血病细胞则没有，这表明HTLV-I与AML的肿瘤发生无关。初次就诊时，通过常规颗粒凝集（PA）试验和间接免疫荧光测定（IF）进行筛查，判断血清抗HTLV-I抗体为阴性。然而，通过蛋白质印迹分析，证明该血清抗HTLV-I抗体呈阳性。这些结果表明，在抗体滴度非常低的极少数情况下，常规PA试验和IF可能会出现假阴性反应。这是关于ATL与另一种类型白血病同时发生的首例报告。
