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与玉米蜡质基因相邻的一个限制性片段长度多态性(RFLP)具有转座元件的结构。

An RFLP adjacent to the maize waxy gene has the structure of a transposable element.

作者信息

Spell M L, Baran G, Wessler S R

机构信息

Botany Department, University of Georgia, Athens 30602.

出版信息

Mol Gen Genet. 1988 Feb;211(2):364-6. doi: 10.1007/BF00330617.

Abstract

Two maize inbred lines harbor non-mutant waxy (Wx) genes that display restriction fragment length polymorphism (RFLP) upstream from the start of Wx transcription. Sequencing of this region in the two strains revealed a DNA insertion with the structural features of a transposable element. The insertion is 316 bp in length, has 15 bp imperfect inverted repeats and is flanked by a 5 bp direct repeat generated upon insertion. Sequences homologous to this insertion are present in multiple copies in maize and its relatives teosinte and Tripsacum but not in the more distantly related dicot tobacco. Finally, this element is not homologous with any previously described maize DNA insertion.

摘要

两个玉米自交系含有非突变的糯性(Wx)基因,这些基因在Wx转录起始位点上游表现出限制性片段长度多态性(RFLP)。对这两个品系的该区域进行测序,发现了一个具有转座元件结构特征的DNA插入片段。该插入片段长度为316 bp,有15 bp的不完全反向重复序列,两侧是插入时产生的5 bp直接重复序列。与该插入片段同源的序列在玉米及其近缘种大刍草和摩擦禾中以多拷贝形式存在,但在亲缘关系较远的双子叶烟草中不存在。最后,该元件与之前描述的任何玉米DNA插入片段均无同源性。

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