Manaharan Thamilvaani, Chakravarthi Srikumar, Radhakrishnan Ammu Kutty, Palanisamy Uma Devi
Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Department of Pathology, Perdana University Graduate School of Medicine, 43400 Selangor, Malaysia.
Toxicol Rep. 2014 Sep 21;1:718-725. doi: 10.1016/j.toxrep.2014.09.006. eCollection 2014.
In this study, the acute and subchronic toxicity effect of the leaf extract (SA) was evaluated. For the acute toxicity study, a single dose of 2000 mg/kg of the SA was given by oral-gavage to male Sprague-Dawley (SD) rats. The rats were observed for mortality and toxicity signs for 14 days. In the subchronic toxicity study the SA was administered orally at doses of 50, 100, and 200 mg/kg per day for 28 days to male SD rats. The animals were sacrificed at the end of the experiment. The parameters measured including food and water intake, body weight, absolute and relative organ weight, blood biochemical tests and histopathology observation. In both the acute and subchronic toxicity studies, SA did not show any visible signs of toxicity. There were also no significant differences between the control and SA treated rats in terms of their food and water intake, body weight, absolute and relative organ weight, biochemical parameters or gross and microscopic appearance of the organs. There were no acute or subchronic toxicity observed and our results indicate that this extract could be devoid of any toxic risk. This is the first study reported the safety and toxicity of SA.
在本研究中，对叶提取物（SA）的急性和亚慢性毒性作用进行了评估。在急性毒性研究中，通过口服灌胃给予雄性斯普拉格-道利（SD）大鼠单剂量2000 mg/kg的SA。观察大鼠14天的死亡率和毒性体征。在亚慢性毒性研究中，将SA以每天50、100和200 mg/kg的剂量口服给予雄性SD大鼠，持续28天。在实验结束时处死动物。测量的参数包括食物和水摄入量、体重、绝对和相对器官重量、血液生化测试以及组织病理学观察。在急性和亚慢性毒性研究中，SA均未显示出任何明显的毒性迹象。在食物和水摄入量、体重、绝对和相对器官重量、生化参数或器官的大体和微观外观方面，对照组和SA处理组的大鼠之间也没有显著差异。未观察到急性或亚慢性毒性，我们的结果表明该提取物可能没有任何毒性风险。这是首次报道SA安全性和毒性的研究。
