Jawaid Sana, Talpur Farah N, Nizamani Shafi M, Afridi Hassan Imran
National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan.
Toxicol Rep. 2015 Oct 21;2:1418-1422. doi: 10.1016/j.toxrep.2015.10.011. eCollection 2015.
Aflatoxin M1 (AFM1) is a potent carcinogen, teratogen and mutagen found in the milk when lactating animals consume feed contaminated with aflatoxin B (AFB). In the present study, the contamination of AFM1 was evaluated in the milk supply chain of the province of Sindh, Pakistan. For the broader profiling of targeted toxin, enzyme-linked immunosorbent assay (ELISA) was used for the determination of AFM1 in both branded and non-branded milk samples. The results showed that 96.43% of samples (81 out of 84) were contaminated with AFM1 in the range of 0.01-0.76 μg/L. The average contamination level was 0.38 μg/L. The determined values of AFM1 in the collected milk samples were above the standard limit of the European Commission while 70% of the samples exceeded levels established by United States regulations. According to these results, the estimated daily intake of AFM1 for adults was determined as 3.1 ng/kg of body weight per day.
黄曲霉毒素M1（AFM1）是一种强效致癌物、致畸物和诱变剂，当泌乳动物食用被黄曲霉毒素B（AFB）污染的饲料时，可在牛奶中发现。在本研究中，对巴基斯坦信德省牛奶供应链中的AFM1污染情况进行了评估。为了更全面地分析目标毒素，采用酶联免疫吸附测定法（ELISA）测定品牌和非品牌牛奶样品中的AFM1。结果显示，96.43%的样品（84份中的81份）被AFM1污染，污染范围为0.01 - 0.76μg/L。平均污染水平为0.38μg/L。所采集牛奶样品中AFM1的测定值高于欧盟委员会的标准限值，而70%的样品超过了美国规定的水平。根据这些结果，确定成年人每天AFM1的估计摄入量为3.1 ng/kg体重。
