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Isolation and sequence of a cDNA clone encoding the 31-kDa subunit of bovine kidney vacuolar H+-ATPase.

作者信息

Hirsch S, Strauss A, Masood K, Lee S, Sukhatme V, Gluck S

机构信息

Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.

出版信息

Proc Natl Acad Sci U S A. 1988 May;85(9):3004-8. doi: 10.1073/pnas.85.9.3004.

DOI:10.1073/pnas.85.9.3004
PMID:2896353
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC280131/
Abstract

We have isolated a cDNA encoding the 31-kDa subunit of the bovine kidney vacuolar H+-ATPase. The composite sequence contains 1219 base pairs, which includes the entire 678-base-pair coding region. A lysine-rich sequence previously found in the Na+, K+-ATPase alpha subunit and the H+,K+-ATPase was identified in the 31-kDa subunit. An RNA blot and an immunoblot demonstrated variable 31-kDa subunit expression and immunoreactivity in different tissues; the highest levels were observed in kidney medulla and brain with both types of analysis. The isolation of a cDNA for the 31-kDa subunit is an important step in understanding this subunit's role in the function and regulation of the vacuolar H+-ATPase.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed3d/280131/3a80575c72a8/pnas00261-0137-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed3d/280131/63ea33065430/pnas00261-0136-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed3d/280131/3a9d981a9581/pnas00261-0136-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed3d/280131/3a80575c72a8/pnas00261-0137-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed3d/280131/63ea33065430/pnas00261-0136-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed3d/280131/3a9d981a9581/pnas00261-0136-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed3d/280131/3a80575c72a8/pnas00261-0137-a.jpg

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Partial purification of a tonoplast ATPase from corn coleoptiles.从玉米幼茎中部分纯化液泡膜 ATP 酶。
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