Dzagurova T K, Tkachenko E A, Petrov V A
Vopr Virusol. 1988 Jan-Feb;33(1):71-5.
Clinical serological studies of hemorrhagic fever with renal syndrome (HFRS) demonstrated high effectiveness of indirect immunofluorescence procedure (IF) for serodiagnosis of this infection. The use of HFRS virus-infected VERO-E6 cells as the antigen for IF enhanced the sensitivity and specificity of the method as compared with the use of cryostatic sections of the lung tissue from wild rodents spontaneously infected with HFRS virus as the antigen. A technology for preparation of a polyvalent HFRS diagnosticum containing the antigens of two virus serotypes has been developed for serological diagnosis of HFRS in the European foci where the serological evidence of circulation of strains of serotypes 1 and 2 of HFRS virus pathogenic for man has been obtained. The specificity and sensitivity of detection of antibody to HFRS virus serotypes 1 and 2 by IF test using the polyvalent diagnosticum is equal to that in tests using individually tissue culture antigens of HFRS virus serotypes 1 and 2. It was found that in the patients' native sera antibodies of the IgM class are masked by antibodies of the IgG fraction, therefore the use of IF technique for detection of IgM antibody to HFRS virus aimed at early serological diagnosis of this infection is not effective.
肾综合征出血热（HFRS）的临床血清学研究表明，间接免疫荧光法（IF）在该感染的血清学诊断中具有高效性。与使用自然感染HFRS病毒的野生啮齿动物肺组织冷冻切片作为抗原相比，使用感染HFRS病毒的VERO-E6细胞作为IF抗原可提高该方法的敏感性和特异性。在欧洲疫区，已获得人致病性HFRS病毒1型和2型毒株传播的血清学证据，为此开发了一种包含两种病毒血清型抗原的多价HFRS诊断制剂技术，用于HFRS的血清学诊断。使用该多价诊断制剂通过IF试验检测HFRS病毒1型和2型抗体的特异性和敏感性，与使用HFRS病毒1型和2型单独的组织培养抗原进行试验的结果相当。研究发现，在患者的天然血清中，IgM类抗体被IgG组分抗体所掩盖，因此，使用IF技术检测HFRS病毒IgM抗体以进行该感染的早期血清学诊断并不有效。
