Zhu Li-Han, Bao Tian-Hui, Deng Yue, Li Hua, Chen Li-Xia
a Key Laboratory of Structure-Based Drug Design & Discovery, School of Traditional Chinese Materia Medica, Wuya College of Innovation, Ministry of Education , Shenyang Pharmaceutical University , Shenyang 110016 , China.
b School of Pharmacy , Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430030, China.
J Asian Nat Prod Res. 2017 Nov;19(11):1079-1086. doi: 10.1080/10286020.2017.1381687. Epub 2017 Oct 3.
A phthalide glycoside, (3R, 4R)-4-O-β-D-glucopyranosyl-senkyunolide (1), and a megastigmane glycoside, (6S, 7R)-3-oxo-megastigma-4, 8-dien-7-O-β-D-glucoside (2), along with two known aglycones (3-4), were isolated from the 70% EtOH extract of fresh whole grass of Apium graveolens L. Their structures were elucidated by extensive spectroscopic analysis. All of these compounds were tested for their inhibitory effects on nitric oxide (NO) production in the RAW 264.7 macrophages. Among them, compounds 3 and 4 showed potent inhibitory activity against LPS-induced nitric oxide production in RAW 264.7 macrophages, with IC values of 24.0 ± 2.1 μM and 28.6 ± 2.4 μM, respectively.
从芹菜（Apium graveolens L.）新鲜全草的70%乙醇提取物中分离出一种苯酞糖苷，(3R, 4R)-4-O-β-D-吡喃葡萄糖基-升麻醇内酯（1）、一种巨大戟烷糖苷，(6S, 7R)-3-氧代-巨大戟-4,8-二烯-7-O-β-D-葡萄糖苷（2），以及两种已知的苷元（3 - 4）。通过广泛的光谱分析阐明了它们的结构。测试了所有这些化合物对RAW 264.7巨噬细胞中一氧化氮（NO）产生的抑制作用。其中，化合物3和4对RAW 264.7巨噬细胞中脂多糖诱导的一氧化氮产生显示出强效抑制活性，IC值分别为24.0 ± 2.1 μM和28.6 ± 2.4 μM。
