BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China.
Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen 518083, China.
Molecules. 2017 Oct 2;22(10):1653. doi: 10.3390/molecules22101653.
Melatonin is a multifunctional bioactive molecule that plays comprehensive physiological roles in all living organisms. -acetylserotonin methyltransferase (ASMT, also known as hydroxyindole -methyltransferase or HIOMT) is the final enzyme for biosynthesis of melatonin. Here, we performed a comparative genomic and transcriptomic survey to explore the family in fish. Two isotypes ( and ) and a new ASMT-like () are all extracted from teleost genomes on the basis of phylogenetic and synteny analyses. We confirmed that C-terminal of the ASMTL proteins (ASMTL-ASMT) is homology to the full length of ASMT1 and ASMT2. Our results also demonstrate that the two isotypes and their distribution in teleosts seem to be the result of combinations of whole-genome duplication (WGD) and gene loss. Differences were also observed in tissue distribution and relative transcript abundances of , and through transcriptomic analysis. Protein sequence alignment and 3D structure prediction of ASMTs and ASMTL suggest differential roles for these genes. In summary, our current work provides novel insights into the genes in fish by combination of genomic and transcriptomic data.
褪黑素是一种多功能生物活性分子，在所有生物中发挥着全面的生理作用。-乙酰血清素甲基转移酶（ASMT，也称为羟基吲哚-甲基转移酶或 HIOMT）是褪黑素生物合成的最后一种酶。在这里，我们进行了比较基因组学和转录组学调查，以探索鱼类中的 家族。两种同工型（和）和一种新的 ASMT 样（）都是基于系统发育和基因同线性分析从硬骨鱼基因组中提取的。我们证实 ASMTL 蛋白的 C 端（ASMTL-ASMT）与 ASMT1 和 ASMT2 的全长同源。我们的结果还表明，两种同工型及其在硬骨鱼中的分布似乎是全基因组复制（WGD）和基因丢失的结果。通过转录组分析，还观察到 、和在组织分布和相对转录丰度上的差异。ASMTs 和 ASMTL 的蛋白序列比对和 3D 结构预测表明这些 基因具有不同的作用。总之，我们的研究工作通过基因组和转录组数据的结合，为鱼类中的 基因提供了新的见解。
