Hu Bo, Zhang Guifang, Liu Wu, Shi Jianmin, Wang Hua, Qi Meifang, Li Jiqin, Qin Peng, Ruan Ying, Huang Hai, Zhang Yijing, Xu Lin
National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant Sciences Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 300 Fenglin Road Shanghai 200032 China.
Pre-National Laboratory for Crop Germplasm Innovation and Resource Utilization Hunan Agricultural University Changsha Hunan 410128 China.
Regeneration (Oxf). 2017 Aug 27;4(3):132-139. doi: 10.1002/reg2.82. eCollection 2017 Jun.
In tissue culture, the formation of callus from detached explants is a key step in plant regeneration; however, the regenerative abilities in different species are variable. While nearly all parts of organs of the dicot are ready for callus formation, mature regions of organs in monocot rice () and other cereals are extremely unresponsive to tissue culture. Whether there is a common molecular mechanism beyond these different regenerative phenomena is unclear. Here we show that the and rice use different regeneration-competent cells to initiate callus, whereas the cells all adopt () and during cell fate transition. Different from which maintains regeneration-competent cells in mature organs, rice exhausts those cells during organ maturation, resulting in regenerative inability in mature organs. Our study not only explains this old perplexity in agricultural biotechnology, but also provides common molecular markers for tissue culture of different angiosperm species.
在组织培养中，从离体外植体形成愈伤组织是植物再生的关键步骤；然而，不同物种的再生能力各不相同。虽然双子叶植物几乎所有器官部位都易于形成愈伤组织，但单子叶植物水稻（）和其他谷物器官的成熟区域对组织培养极不敏感。这些不同再生现象背后是否存在共同的分子机制尚不清楚。在这里，我们表明拟南芥和水稻利用不同的具有再生能力的细胞来启动愈伤组织形成，而这些细胞在细胞命运转变过程中均采用WUSCHEL（WUS）和CLAVATA3（CLV3）。与拟南芥在成熟器官中维持具有再生能力的细胞不同，水稻在器官成熟过程中耗尽了这些细胞，导致成熟器官无法再生。我们的研究不仅解释了农业生物技术中这一由来已久的困惑，还为不同被子植物物种的组织培养提供了共同的分子标记。
