Blaskett A C, Cox J C
Bacteriology R & D, Commonwealth Serum Laboratories, Parkville, Australia.
Epidemiol Infect. 1988 Jun;100(3):335-44. doi: 10.1017/s095026880006708x.
The predominant causative organism of whooping cough in Australia is of a serotype which has normally been associated overseas with unvaccinated communities. Australian DTP vaccines pass the statutory mouse test for Bordetella pertussis potency but this test is now believed to be relatively insensitive to certain factors, especially the major type-specific agglutinogens, which are presumably also important in the human host-parasite relationship. Because endemic B. bronchiseptica infections make some laboratory animals unsatisfactory for testing B. pertussis agglutinin responses, we have developed a test in which young farm sheep were immunized with vaccines. Type-specific agglutinins in their sera were assayed after absorption of non-specific agglutinins by suspensions of selected bordetella strains. Three well-reputed European DTP vaccines and two recent batches of Australian DTP vaccine were tested and compared thus. All evoked significant agglutinin responses to the main agglutinogens.
在澳大利亚，百日咳的主要致病病原体是一种血清型，在海外通常与未接种疫苗的群体有关。澳大利亚的白喉破伤风百日咳（DTP）疫苗通过了针对百日咳博德特氏菌效力的法定小鼠试验，但现在认为该试验对某些因素相对不敏感，尤其是主要的型特异性凝集原，而这些凝集原在人类宿主与寄生虫的关系中可能也很重要。由于地方性支气管败血博德特氏菌感染会使一些实验动物不适合用于检测百日咳博德特氏菌的凝集素反应，我们开发了一种用疫苗免疫幼龄农场绵羊的试验。在通过选定的博德特氏菌菌株悬液吸收非特异性凝集素后，检测它们血清中的型特异性凝集素。由此对三种声誉良好的欧洲DTP疫苗和两批近期的澳大利亚DTP疫苗进行了测试和比较。所有疫苗都引发了对主要凝集原的显著凝集素反应。
