Ashworth L A, Fitzgeorge R B, Irons L I, Morgan C P, Robinson A
J Hyg (Lond). 1982 Jun;88(3):475-86. doi: 10.1017/s0022172400070339.
Two Bordetella pertussis antigen preparations, outer membrane protein (OMP) and filamentous haemagglutinin (FHA), and a standard vaccine were used to immunize rabbits, and the effects on nasopharyngeal colonization by the organism were determined. Antibodies were measured in serum and in nasal washes by ELISA before and after challenge of the rabbits with 10(6) bacteria of strain M2. Recoveries of B. pertussis in nasal washes were used to assess colonization, which in controls persisted for at least 65 days. Some rabbits of all the immunized groups showed enhanced clearance, but there was no correlation between the elimination of B. pertussis and serum antibodies to OMP, FHA, lipopolysaccharide, lymphocytosis-promoting factor or agglutinogen 3. In contrast, nasal IgA antibody to FHA showed significant inverse correlation with bacterial persistence. Such antibody was induced by the OMP preparation as well as by FHA, but to different extents depending on the immunization schedule and adjuvant used.
使用两种百日咳博德特氏菌抗原制剂，即外膜蛋白（OMP）和丝状血凝素（FHA），以及一种标准疫苗对兔子进行免疫，并测定其对该生物体在鼻咽部定植的影响。在用M2菌株的10(6)个细菌攻击兔子之前和之后，通过ELISA测定血清和鼻腔灌洗液中的抗体。鼻腔灌洗液中百日咳博德特氏菌的回收率用于评估定植情况，在对照组中这种定植持续至少65天。所有免疫组的一些兔子显示出清除能力增强，但百日咳博德特氏菌的清除与针对OMP、FHA、脂多糖、淋巴细胞增多促进因子或凝集原3的血清抗体之间没有相关性。相反，针对FHA的鼻腔IgA抗体与细菌持续存在呈显著负相关。这种抗体由OMP制剂以及FHA诱导产生，但根据免疫方案和所用佐剂的不同，诱导程度有所不同。
