Grace Nirmala J, Evangeline Celsia S, Swaminathan Akila, Narendhirakannan R T, Chatterjee Suvro
Department of Biotechnology, School of Biotechnology and Health Sciences, Karunya University (Karunya Institute of Technology and Sciences), Karunya Nagar, Coimbatore, Tamil Nadu, 641 114, India.
AU-KBC Research Centre and Department of Biotechnology, Anna University, Chennai, India.
Cytotechnology. 2018 Apr;70(2):537-554. doi: 10.1007/s10616-017-0125-0. Epub 2017 Oct 5.
Vitis vinifera. L is one of the most widely consumed fruits in the world and are rich in antioxidant abundant polyphenols. The present study was carried out to assess the antiproliferative and apoptotic effects of Vitis vinifera peel and seed extracts in an in vitro model using human epidermoid carcinoma A431 cell lines. Vitis vinifera peel and seed extracts were incubated with A431 cells to evaluate the antiproliferative, apoptotic effects and the morphological apoptotic changes induced by the extracts. Mitochondrial membrane potential was also measured after incubating the cells with extracts. At the inhibitory concentration (IC), grape seed extract (111.11 µg/mL) and grape peel extract (319.14 µg/mL) were incubated for 24 h with A431 cells. Vitis vinifera peel and seed extracts were able to impart cytotoxic effects, induced apoptosis and apoptotic morphological changes in A431 cells significantly (p < 0.01) and this effect is associated with the interference with mitochondrial membrane potential. This reduction in mitochondrial membrane potential probably initiated the apoptotic cascade in the extracts treated cells. Vitis vinifera peel and seed phytochemicals can selectively target cancer cells and the phytochemicals that are occluded can serve as potential anticancer agents providing better efficacy in killing cancer cells.
葡萄（Vitis vinifera. L）是世界上消费最为广泛的水果之一，富含具有抗氧化作用的丰富多酚类物质。本研究旨在利用人表皮样癌A431细胞系，在体外模型中评估葡萄皮和葡萄籽提取物的抗增殖和凋亡作用。将葡萄皮和葡萄籽提取物与A431细胞共同孵育，以评估提取物诱导的抗增殖、凋亡作用以及形态学凋亡变化。在用提取物孵育细胞后，还测量了线粒体膜电位。在抑制浓度（IC）下，葡萄籽提取物（111.11 µg/mL）和葡萄皮提取物（319.14 µg/mL）与A431细胞孵育24小时。葡萄皮和葡萄籽提取物能够显著（p < 0.01）对A431细胞产生细胞毒性作用、诱导凋亡及凋亡形态学变化，且这种作用与对线粒体膜电位的干扰有关。线粒体膜电位的这种降低可能启动了提取物处理细胞中的凋亡级联反应。葡萄皮和葡萄籽中的植物化学物质可以选择性地靶向癌细胞，其中所含的植物化学物质可作为潜在的抗癌剂，在杀死癌细胞方面具有更好的疗效。
