Centre for Research in Biotechnology for Agriculture (CEBAR), Universiti Malaya, 50603, Kuala Lumpur, Malaysia.
Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603, Kuala Lumpur, Malaysia.
Antonie Van Leeuwenhoek. 2022 Aug;115(8):995-1007. doi: 10.1007/s10482-022-01750-8. Epub 2022 Jun 8.
In this study, a novel bacterium designated F3b2 was isolated from the gut sample of weaver ant Oecophylla smaragdina and characterised. Strain F3b2 was a Gram-negative, aerobic, non-motile, ovoid-shaped bacterium and grows optimally at 28-30 °C. Its major respiratory quinone is ubiquinone 10 (Q-10) and the major fatty acids are C ω7c, C cyclo ω8c and C, representing 85% of the total fatty acids. The 16S rRNA gene sequence of strain F3b2 was highest in similarity to that of Oecophyllibacter saccharovorans DSM106907 and Swingsia samuieinsis NBRC 107927 at 94.35% and 91.96%, respectively. A 16S rRNA gene-based phylogenetic analysis and a core genes-based phylogenomic analysis placed strain F3b2 in a distinct lineage in the family Acetobacteraceae. The phylogenetic placement was supported by lower than species delineation threshold average nucleotide identity (ANI) (≤ 70.2%), in silico DNA-DNA hybridization (DDH) (≤ 39.5%) and average amino acid identity (AAI) (≤ 63.5%) values between strain F3b2 and closest neighbours. These overall genome relatedness indices also supported the assignment of strain F3b2 to a novel genus within Acetobacteraceae. The genome of strain F3b2 was 1.96 Mb with 60.4% G + C DNA content. Based on these results, strain F3b2 represented a novel taxon of Acetobacteraceae, for which we proposed the name Formicincola oecophyllae gen. nov. sp. nov., and strain F3b2 (= LMG 30590 = DSM 106908 = NBRC 113640 = KCTC 62951) as the type strain.
在这项研究中，从绿巨蚁 Oecophylla smaragdina 的肠道样本中分离到一株新型细菌 F3b2 并对其进行了表征。F3b2 菌株为革兰氏阴性、需氧、非运动、卵形细菌，最适生长温度为 28-30°C。其主要呼吸醌为泛醌 10（Q-10），主要脂肪酸为 C ω7c、C 环 ω8c 和 C，占总脂肪酸的 85%。菌株 F3b2 的 16S rRNA 基因序列与 Oecophyllibacter saccharovorans DSM106907 和 Swingsia samuieinsis NBRC 107927 的相似度最高，分别为 94.35%和 91.96%。基于 16S rRNA 基因的系统发育分析和基于核心基因的系统基因组分析将菌株 F3b2 置于醋酸杆菌科的一个独特谱系中。这种系统发育位置得到了低于种间区分阈值的平均核苷酸同一性（ANI）（≤70.2%）、体外 DNA-DNA 杂交（DDH）（≤39.5%）和平均氨基酸同一性（AAI）（≤63.5%）值的支持，这些值在菌株 F3b2 和最接近的近缘种之间。这些全基因组相关指数也支持将菌株 F3b2 归类为醋酸杆菌科中的一个新属。菌株 F3b2 的基因组为 1.96 Mb，G+C DNA 含量为 60.4%。基于这些结果，菌株 F3b2 代表了醋酸杆菌科的一个新分类群，我们提议将其命名为 Formicincola oecophyllae gen. nov. sp. nov.，菌株 F3b2（=LMG 30590=DSM 106908=NBRC 113640=KCTC 62951）为模式菌株。
