State Key Laboratory of Agrobiotechnology, Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing, 100193, People's Republic of China.
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
Antonie Van Leeuwenhoek. 2021 Apr;114(4):355-364. doi: 10.1007/s10482-021-01516-8. Epub 2021 Feb 6.
A Gram-staining-negative, non-motile, aerobic bacterium, designated 1-3, was isolated from oil reservoir water collected from Liaohe oilfield, north-east of China. Growth was observed at 15-40 °C (optimum 37 °C) and pH 6-10 (optimum 7). The strain can grow under nitrogen-limiting condition. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel isolate was most closely related to Siccirubricoccus deserti SYSU D8009 (96.7%), followed by Paracraurococcus ruber NS89 (95.7%) and Belnapia rosea CPCC 100156 (94.9%). Genome sequencing revealed a genome size of 6.43 Mbp and a G+C content of 71.3 mol%. The average nucleotide identity values and digital DNA-DNA hybridization between 1-3 and the reference strains were all below the cut-off level (95-96% and 70%, respectively) for species delineation. The strain possessed the cytochrome P450 enzyme, which has the potential to degrade oil. The respiratory quinone was Q-10 and the major fatty acids were summed feature 8 (C ω7c/C ω6c, 38.8%), C (25.6%) and C cyclo ω8c (22.5%). The polar lipids of strain 1-3 comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and three unidentified aminolipids. Based on the genotypic and phenotypic characteristics, strain 1-3 represents a novel species of genus Siccirubricoccus, for which the name Siccirubricoccus phaeus sp. nov. is proposed. The type strain of Siccirubricoccus phaeus is 1-3 (= CGMCC 1.16799 = LMG 31398).
一株革兰氏染色阴性、非运动性、需氧细菌，被命名为 1-3，从中国东北辽河油田的油藏水中分离得到。该菌在 15-40°C（最佳温度 37°C）和 pH 6-10（最佳 pH 7）条件下生长。该菌株可在氮限制条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，新分离株与 Siccirubricoccus deserti SYSU D8009（96.7%）最为密切相关，其次为 Paracraurococcus ruber NS89（95.7%）和 Belnapia rosea CPCC 100156（94.9%）。基因组测序显示，其基因组大小为 6.43 Mbp，G+C 含量为 71.3 mol%。1-3 与参考菌株之间的平均核苷酸同一性值和数字 DNA-DNA 杂交值均低于种间划分的界限（分别为 95-96%和 70%）。该菌株具有细胞色素 P450 酶，具有降解石油的潜力。呼吸醌为 Q-10，主要脂肪酸为 summed feature 8（C ω7c/C ω6c，38.8%）、C（25.6%）和 C cyclo ω8c（22.5%）。菌株 1-3 的极性脂包括双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱和三种未鉴定的氨基脂。基于基因型和表型特征，菌株 1-3 代表 Siccirubricoccus 属的一个新种，命名为 Siccirubricoccus phaeus sp. nov.。Siccirubricoccus phaeus 的模式菌株为 1-3（= CGMCC 1.16799 = LMG 31398）。
