Pseudomonas tumuqii sp. nov., isolated from greenhouse soil.

A Gram-stain-negative, aerobic, rod-shaped and motile bacterium, named LAMW06, was isolated from greenhouse soil in Beijing, China. In the 16S rRNA gene sequence comparison, strain LAMW06 had the highest similarity with Pseudomonas cuatrocienegasensis 1N. Phylogenetic analysis based on the 16S rRNA and three housekeeping gene sequences (gyrB, rpoB and rpoD) indicated that strain represented a member of the genus Pseudomonas. The genome sequence size of the isolate was 5.5 Mb, with a DNA G + C content of 63.5 mol%. The average nucleotide identity and DNA-DNA hybridization values between strain LAMW06 and closely related members of Pseudomonas borbori R-20821, Pseudomonas taeanensis MS-3 and P. cuatrocienegasensis 1N were 90.9%, 82.4%, 81.5% and 43.0%, 25.9%, 24.6% respectively. The major fatty acids contained summed feature 3 (C ω6c and/or C ω7c), C ω7c and C. The primary respiratory quinone was ubiquinone-9. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, six aminophospholipids, six phospholipids, one aminolipid and one glycolipid. According to the genotypic, phylogenetic and chemotaxonomic data, strain LAMW06 represents a novel species within the genus Pseudomonas, for which the name Pseudomonas tumuqii sp. nov. is proposed. The type strain is LAMW06 (= GDMCC 1.2003 = KCTC 72829).