Gibbs Ebrima, Matsubara Joanne, Cao Sijia, Cui Jing, Forooghian Farzin
NeuroImmunology/NeuroProteomics Laboratories, Brain Research Centre, University of British Columbia, Vancouver, BC.
Department of Ophthalmology, University of British Columbia, Vancouver, BC.
Can J Ophthalmol. 2017 Oct;52(5):463-467. doi: 10.1016/j.jcjo.2017.03.010. Epub 2017 May 10.
Antiretinal antibodies (ARAs) have previously been described in noninfectious uveitis. However, the antigen specificity of these ARAs has not been investigated. The purpose of this study was to identify antigen-specific ARAs in noninfectious uveitis.
A total of 18 patients with noninfectious uveitis were enrolled. Surface plasmon resonance was used to measure binding responses of patient and control sera against several uveitogenic proteins: recoverin, S-antigen, interphotoreceptor retinoid binding (IRBP), retinal-pigment-epithelium-specific 65-kDa protein (RPE65), tyrosinase-related protein 1 (TRYP1), and tyrosinase-related protein 2 (TRYP2).
The frequency of ARA positivity against S-antigen, IRBP, RPE65, TYRP1, and TYRP2 in patients with uveitis did not differ significantly from that of normal controls. However, ARA positivity for recoverin was more frequently observed in patients with uveitis (p = 0.002). A total of 10 patients in the uveitis cohort had birdshot chorioretinopathy, and all 10 were positive for anti-recoverin ARAs.
Patients with noninfectious uveitis have increased frequency of ARA positivity against recoverin. This ARA deserves further investigations as a potential biomarker and pathogenic agent in noninfectious uveitis, especially in birdshot chorioretinopathy.
抗视网膜抗体（ARA）此前已在非感染性葡萄膜炎中被描述。然而，这些ARA的抗原特异性尚未得到研究。本研究的目的是鉴定非感染性葡萄膜炎中抗原特异性的ARA。
共纳入18例非感染性葡萄膜炎患者。采用表面等离子体共振技术测量患者血清和对照血清对几种葡萄膜炎致病蛋白的结合反应：恢复蛋白、S抗原、光感受器间类视黄醇结合蛋白（IRBP）、视网膜色素上皮特异性65 kDa蛋白（RPE65）、酪氨酸酶相关蛋白1（TRYP1）和酪氨酸酶相关蛋白2（TRYP2）。
葡萄膜炎患者中针对S抗原、IRBP、RPE65、TYRP1和TYRP2的ARA阳性频率与正常对照组无显著差异。然而，葡萄膜炎患者中恢复蛋白的ARA阳性更为常见（p = 0.002）。葡萄膜炎队列中有10例患者患有鸟枪弹样脉络膜视网膜病变，所有10例患者的抗恢复蛋白ARA均为阳性。
非感染性葡萄膜炎患者中针对恢复蛋白的ARA阳性频率增加。这种ARA作为非感染性葡萄膜炎，尤其是鸟枪弹样脉络膜视网膜病变的潜在生物标志物和致病因子，值得进一步研究。
