Guo Yaning, Pang Chaoyou, Jia Xiaoyun, Ma Qifeng, Dou Lingling, Zhao Fengli, Gu Lijiao, Wei Hengling, Wang Hantao, Fan Shuli, Su Junji, Yu Shuxun
College of Agronomy, Northwest A&F UniversityYangling, China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural SciencesAnyang, China.
Front Plant Sci. 2017 Sep 25;8:1657. doi: 10.3389/fpls.2017.01657. eCollection 2017.
Plant-specific NAC proteins comprise one of the largest transcription factor families in plants and play important roles in plant development and the stress response. L. is a major source of fiber, but its growth and productivity are limited by many biotic and abiotic stresses. In this study, the NAC domain gene was functionally characterized in detail, and according to information about the cotton genome sequences, it was located on scaffold42.1, containing three exons and two introns. Promoter analysis indicated that the promoter contained both basic and stress-related elements, and it was especially expressed in the cotyledon of A transactivation assay in yeast demonstrated that was a transcription activator, and its activation domain was located at its C-terminus. The results of qRT-PCR proved that was preferentially expressed at later stages of cotyledon and fiber development, and it showed high sensitivity to ethylene and meJA treatments. Overexpression of resulted in an early flowering phenotype in , and it also improved drought tolerance in both and cotton. Furthermore, VIGS-induced silencing of in cotton led to a drought-sensitive phenotype. In summary, positively regulates drought stress, and it also responds to ethylene and meJA treatments, making it a candidate gene for stress studies in cotton.
植物特有的NAC蛋白是植物中最大的转录因子家族之一，在植物发育和应激反应中发挥重要作用。陆地棉是纤维的主要来源，但其生长和生产力受到许多生物和非生物胁迫的限制。在本研究中，对NAC结构域基因进行了详细的功能鉴定，并根据棉花基因组序列信息，将其定位在scaffold42.1上，包含三个外显子和两个内含子。启动子分析表明，该基因启动子同时包含基本元件和胁迫相关元件，且在陆地棉子叶中特异性表达。酵母中的反式激活分析表明，该基因是一种转录激活因子，其激活结构域位于C末端。qRT-PCR结果证明，该基因在子叶和纤维发育后期优先表达，且对乙烯和茉莉酸甲酯处理表现出高敏感性。该基因的过表达导致拟南芥出现早花表型，同时也提高了拟南芥和棉花的耐旱性。此外，在棉花中通过VIGS诱导沉默该基因导致出现干旱敏感表型。总之，该基因正向调控干旱胁迫，且对乙烯和茉莉酸甲酯处理有响应，使其成为棉花胁迫研究的候选基因。
